唾液ANG-2浓度和口腔扁平苔藓炎症活跃程度的关联性分析

doi:10.13591/j.cnki.kqyx.2025.01.007

口腔医学 ›› 2025, Vol. 45 ›› Issue (1): 45-50.doi: 10.13591/j.cnki.kqyx.2025.01.007

唾液ANG-2浓度和口腔扁平苔藓炎症活跃程度的关联性分析

向远燚¹, 罗晶晶², 李海洋¹^,³, 吴中婷¹^,⁴(), 周红梅¹()

1 口腔疾病防治全国重点实验室,国家口腔医学中心,国家口腔疾病临床医学研究中心,“口腔医学+”前沿医学创新中心,四川大学华西口腔医院口腔黏膜病科,四川成都(610041)
2 口腔疾病防治全国重点实验室,国家口腔医学中心,国家口腔疾病临床医学研究中心,“口腔医学+”前沿医学创新中心,四川大学华西口腔医院预防口腔科,四川成都(610041)
3 重庆医科大学附属口腔医院大学城门诊部口腔科,重庆(400015)
4 攀枝花市攀钢集团总医院口腔科,四川攀枝花(617000)

收稿日期:2024-05-10 出版日期:2025-01-28 发布日期:2025-01-16
通讯作者: 周红梅 E-mail:zhouhm@scu.edu.cn; 吴中婷 E-mail:540327390@qq.com
基金资助:
国家自然科学基金(81272962);国家自然科学基金(82071124);国家自然科学基金(82301094);四川大学华西口腔医院探索与研发项目(RD-03-202410)

Correlation between salivary ANG-2 level and inflammation activity of oral lichen planus

XIANG Yuanyi¹, LUO Jingjing², LI Haiyang¹^,³, WU Zhongting¹^,⁴(), ZHOU Hongmei¹()

State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Frontier Innovation Center for Dental Medicine Plus, Department of Oral Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2024-05-10 Online:2025-01-28 Published:2025-01-16

摘要/Abstract

摘要：

目的检测口腔扁平苔藓(oral lichen planus,OLP)患者的血管生成素-2(angiopoietin 2,ANG-2)唾液浓度,分析其与OLP炎症活跃程度的关联性。方法纳入89例OLP患者,分为无症状非糜烂型组、有症状非糜烂型组、轻中度糜烂型组及重度糜烂型组,15名健康成人作为正常对照组。收集受试者非刺激性全唾液,采用化学发光免疫法检测ANG-2浓度并进行分析。收集正常口腔黏膜组织和非糜烂型、糜烂型OLP口腔黏膜组织,采用免疫组化染色检测间质血管ANG-2表达。结果与正常对照组相比,OLP组年龄和性别均无统计学差异,OLP全组及非糜烂型、糜烂型OLP组的唾液ANG-2浓度均显著增高(P<0.05);其中,糜烂型OLP组显著高于非糜烂型(P=0.022),有症状非糜烂型组高于无症状非糜烂型组(P=0.048),糜烂型亚组之间无统计学差异(P=0.067)。Spearman相关性分析显示,唾液ANG-2浓度与OLP患者炎症活跃程度呈正相关(r=0.314,P=0.003)。非糜烂型OLP间质血管的ANG-2表达较正常黏膜组织增加,但无统计学意义(P>0.05);糜烂型OLP间质血管ANG-2表达较正常黏膜组织(P<0.001)及非糜烂型OLP(P<0.001)均显著增加。结论唾液ANG-2浓度与OLP炎症活跃程度有较高关联性,提示其可作为OLP炎症活跃标志物之一,辅助临床无创监测OLP病情变化。

关键词: 口腔扁平苔藓, 炎症程度, 血管生成素-2, 唾液, 无创监测

Abstract:

Objective To assay the salivary ANG-2 level of oral lichen planus (OLP) patients, and analyze its correlation with inflammatory activity of OLP. Methods Eighty-nine OLP patients were included, and divided into four subgroups as non-erosive asymptomatic (NEA), non-erosive symptomatic (NES), minor-erosive (MIE) and major-erosive (MAE) groups. Fifteen healthy adults were recruited as controls. Whole unstimulated saliva was collected from each participant, and the salivary ANG-2 level was measured by chemiluminescence immunoassays (CLIA) for analysis. Normal oral mucosal tissue, non-erosive and erosive OLP tissues were collected to detect and analyze the expression of ANG-2 positive blood vessels by immunohistochemistry (IHC). Results The baselines of age and gender between OLP and control groups showed no significant difference. Compared to controls, the salivary ANG-2 levels of OLP group, non-erosive and erosive OLP subgroups were significantly higher (P<0.05), in which erosive OLP group was higher than non-erosive OLP group (P=0.022); NES subgroup was slightly higher than NEA (P=0.048), and there was no statistical significance between MIA and MEA subgroups (P=0.067). Spearman correlation analysis showed a positive correlation between salivary ANG-2 level and inflammation activity in OLP patients (r=0.314,P=0.003). The expression of ANG-2 in non-erosive OLP mucosal tissues slightly increased than normal oral mucosal tissue (P>0.05), but there was no significant difference. The expression of ANG-2 in erosive OLP mucosal tissues significantly increased than normal oral mucosal tissue (P<0.001) and non-erosive OLP group (P<0.001). Conclusion There is a certain correlation between salivary ANG-2 level and inflammatory activity of OLP, indicating that salivary ANG-2 level is probable to be one of the inflammatory activity indicators to monitor the state-variation of OLP as a clinical non-invasive method.

Key words: oral lichen planus, inflammatory activity, angiopoietin 2, saliva, non-invasive monitoring

中图分类号:

R363.2

向远燚, 罗晶晶, 李海洋, 吴中婷, 周红梅. 唾液ANG-2浓度和口腔扁平苔藓炎症活跃程度的关联性分析[J]. 口腔医学, 2025, 45(1): 45-50.

XIANG Yuanyi, LUO Jingjing, LI Haiyang, WU Zhongting, ZHOU Hongmei. Correlation between salivary ANG-2 level and inflammation activity of oral lichen planus[J]. Stomatology, 2025, 45(1): 45-50.

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参考文献 23

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组别	年龄/岁		性别				病程/月
组别	年龄/岁		男		女		病程/月
NEA OLP组		41.59±11.81		8		14		24.53±19.40
NES OLP组		44.40±12.41		7		18		8.21±6.11
MIE OLP组		54.71±11.71		3		18		12.17±10.64
MAE OLP组		53.38±13.75		4		17		15.34±13.32
正常对照组		36.20±9.97		5		10		0

唾液ANG-2浓度和口腔扁平苔藓炎症活跃程度的关联性分析

Correlation between salivary ANG-2 level and inflammation activity of oral lichen planus

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