环状RNA-BIRC6促进血管新生的作用研究

doi:10.13591/j.cnki.kqyx.2022.06.005

Abstract

Abstract: Objective To analyze the differentially expressed circBIRC6 in human umbilical vein endothelial cells (HUVEC) induced by conditioned medium of human amniotic mesenchymal stem cell (hAMSC-CM) and its role in angiogenesis. Methods The expression of circBIRC6 in HUVEC was tested by qRT-PCR. Si-circBIRC6 and circBIRC6-overexpressed plasmid were made to construct cell models of knockdown and overexpressed circBIRC6 by transfection. The angiogenesis ability of HUVEC was detected by Transwell migration assay, tube formation assay and wound healing assay. Results hAMSC-CM induced the expression of circBIRC6 in HUVEC. qRT-PCR verified the reliability of HUVEC knockdown and overexpression cell model. In Transwell migration assay, tube formation assay and wound healing assay, the knockdown of circBIRC6 reduced cell mobility, total tube length and scratch mobility of HUVEC, while overexpression of circBIRC6 increased them. Conclusion This study elucidates the positive role of circBIRC6 in HUVEC angiogenesis and reveals that hAMSC-CM may promote HUVEC angiogenesis by up-regulating circBIRC6.

Key words: human amniotic mesenchymal stem cell, human umbilical vein endothelial cells, angiogenesis, circBIRC6

CLC Number:

R329.2

SUN Dian, XIAO Yijing, SHEN Ming. Study on the effect of circBIRC6 on promoting angiogenesis[J]. Stomatology, 2022, 42(6): 508-514.

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Study on the effect of circBIRC6 on promoting angiogenesis

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