Stomatology ›› 2022, Vol. 42 ›› Issue (12): 1057-1062.doi: 10.13591/j.cnki.kqyx.2022.12.001

• Basic Research •     Next Articles

Effect of remineralization procedures on surface morphology and Ca/P ratio of bleached enamel

WANG Jingchan, LI Yanping, HE Lina, NIU Yumei   

  1. Department of Endodontics, School of Stomatology, Harbin Medical University, Department of Endodontics, the First Affiliated Hospital of Harbin Medical,Harbin 150001,China
  • Received:2022-05-16 Online:2022-12-28 Published:2022-12-28

Abstract: Objective To study the effect of remineralization procedures on surface morphology and Ca/P of bleached enamel. Methods Enamel samples of bovine teeth were prepared and bleached with bleaching gel, and then randomly divided into five groups for different remineralization procedures:deionized water (DW) group; 2% NaF group; 10% procyanidins (PC) group; casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) group; PC+CPP-ACP group. Changes of color, surface morphology, elements and roughness were detected by chromometer, scanning electron microscopy, energy dispersive spectroscopy and atomic force microscopy before bleaching, immediately after bleaching and one week after remineralization, respectively. Results Significant differences in ΔE were not found among bleached groups (P>0.05). Mineral sediments of different degrees could be observed in all groups after remineralization procedures, especially in PC+CPP-ACP group. Energy dispersive spectroscopy showed that bleaching significantly reduced the Ca/P ratio (P<0.05). All remineralization treatment increased the Ca/P ratio. NaF and PC+CPP-ACP exhibited higher remineralization capacity in comparison to other groups (P<0.05). Atomic force microscopy showed that mineral sediments were observed and roughness was reduced in all groups after remineralization (P<0.05), and sediments were more obvious and roughness was much smaller in PC+CPP-ACP group (P<0.05). Conclusion PC and CPP-ACP could promote the remineralization on enamel surface after bleaching, and their combined effect was better. PC and CPP-ACP did not interfere with bleaching efficacy.

Key words: procyanidins, casein phosphopeptide-amorphous calcium phosphate, remineralization, bleaching

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