PAAc-NHS酯/明胶复合水凝胶口腔贴剂的性能研究

doi:10.13591/j.cnki.kqyx.2023.04.003

Abstract

Abstract:

Objective To compare the effects of different levels of poly(acrylic acid)grafted with N-hydrosuccinimide ester(PAAc-NHS ester)on the mechanical properties and in vitro adhesion properties of PAAc-NHS ester/gelatin composite hydrogels. Methods The PAAc-NHS ester/gelatin composite hydrogels were constructed via ultraviolet cross-linking method and divided into three groups by mass fraction of PAAc-NHS ester:A(10%), B(20%)and C(30%). Scanning electron microscopy(SEM) was used to observe the surface morphology of the composite hydrogels, and Fourier infrared spectroscopy(FT-IR)was used to detect the surface characteristic functional groups of PAAc-NHS ester/gelatin composite hydrogels; mechanical properties of hydrogel were tested by tensile test; the swelling ratio and water retention ratio of the composite hydrogels were calculated and the adhesive properties and cytotoxicity of hydrogels were evaluated. Results SEM results showed that PAAc-NHS ester/gelatin composite hydrogel had porous microstructure, and the pore size decreased with the increase of PAAc-NHS ester content; the FT-IR results showed that PAAc-NHS ester was cross-linked with gelatin in all groups; the tensile results revealed that Group C had the strongest mechanical properties (tensile stress was (140.15±45.24) kPa; elongation at break was (310.19±11.43)%); the swelling and water retention test results showed that group A had the highest water absorption and water retention rates; in vitro adhesion experiments showed that all three groups of PAAc-NHS ester/gelatin composite hydrogels had good adhesion properties in vitro and showed no cytotoxicity. Conclusion All the groups of PAAc-NHS ester/gelatin composite hydrogels have good mechanical properties and adhesion in vitro, and have no cytotoxicity. Therefore, they have the potential to be applied as oral patches.

Key words: hydrogel, polyacrylic acid, oral patch, wound dressings

CLC Number:

R783.1

DUN Zhiyue,SUN Chuanyue,ZHANG Zhihao,SHU Fei,ZHANG Qinghong,LIU Mei. Study on properties of PAAc-NHS ester/gelatin composite oral patches[J]. Stomatology, 2023, 43(4): 301-307.

Figures/Tables 9

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References 24

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组别	拉伸强度/kPa	断裂伸长率/%
A	54.54±21.39	130.93±54.09
B	73.83±22.15	217.92±75.16
C	140.15±45.24^*	310.19±11.43^*

Study on properties of PAAc-NHS ester/gelatin composite oral patches

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组别	丙烯酸/%	AAc-NHS酯/%	明胶/%	gelMA/%	α-酮戊二酸/%	去离子水/%
A	10	0.33	10	0.1	0.2	79.37
B	20	0.67	10	0.1	0.2	69.03
C	30	1.00	10	0.1	0.2	58.70

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