不同程度颈动脉狭窄患者动脉粥样硬化斑块中牙周致病菌的分布研究

doi:10.13591/j.cnki.kqyx.2024.01.007

Abstract

Abstract:

Objective To detect the distribution of periodontal pathogens in atherosclerotic plaque and subgingival plaque in patients with different degrees of carotid artery stenosis. Methods Fifty patients requiring carotid endarterectomy were selected and divided into severe stenosis group (36 cases) and complete occlusion group (14 cases) according to the degree of carotid artery stenosis. The distribution of five periodontal pathogens Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), Tanerella forsythia (T. forsythia),Treponema denticola (T. denticola), Fusobacterium nucleatum (F. nucleatum), Porphyromonas gingivalis (P. gingivalis) was detected by 16S rRNA PCR. Results The detection rates of P. gingivalis, T. denticola,T. forsythia and F.nucleatum in carotid atherosclerotic plaques in patients with complete occlusion were 71.4%, 64.3%, 42.9% and 14.3%, which were higher than those in patients with severe stenosis, and there were significant differences in the detection rates of P.gingivalis and T.denticola(P<0.05). A.actinomycetemcomitans was not detected in the plaques of the complete occlusion group, which was lower than that in the severe stenosis group, but the difference was not statistically significant. The detection rates of P. gingivalis, T. denticola and T. forsythia in subgingival plaque in patients with complete occlusion were 85.7%, 71.4% and 64.3%, which were also higher than those in patients with severe stenosis, but there was no statistical significance (P>0.05). In addition, the detection rates of P.gingivalis, T.denticola, and T.forsythia in carotid atherosclerotic plaques and subgingival plaques were significantly higher than those of F.nucleatum and A.actinomycetemcomitans (P<0.05). Conclusion The distribution of P.gingivalis, A.actinomycetemcomitans, F.nucleatum, T.denticola, and T.forsythia is different in carotid atherosclerotic plaque and subgingival plaque. The detection rates of P.gingivalis and T.denticola in carotid atherosclerotic plaques in patients with total carotid artery occlusion are significantly higher, which may be closely related to the severity of carotid atherosclerosis.

Key words: carotid atherosclerosis, periodontal pathogens, periodontitis, subgingival plaque, plaque

CLC Number:

R54
R781.4

TANG Lu, XUE Dong, YANG Wenwen, ZHANG Xianli, LU Xia, ZHAO Ying. Distribution of periodontal pathogens in carotid atherosclerotic plaques with different degrees of carotid artery stenosis[J]. Stomatology, 2024, 44(1): 31-35.

Figures/Tables 4

Tab.1

Fig.1

Tab.2

Tab.3

References 31

[1]	Donkor ES. Stroke in the 21^st century:A snapshot of the burden, epidemiology, and quality of life[J]. Stroke Res Treat, 2018, 2018:3238165.
[2]	Flaherty ML, Kissela B, Khoury JC, et al. Carotid artery stenosis as a cause of stroke[J]. Neuroepidemiology, 2013, 40(1):36-41. doi: 10.1159/000341410 pmid: 23075828
[3]	Lamprecht R, Rimmele DL, Schnabel RB, et al. Cross-sectional analysis of the association of periodontitis with carotid intima media thickness and atherosclerotic plaque in the Hamburg City health study[J]. J Periodontal Res, 2022, 57(4):824-834. doi: 10.1111/jre.13021 pmid: 35675038
[4]	Paul O, Arora P, Mayer M, et al. Inflammation in periodontal disease:Possible link to vascular disease[J]. Front Physiol, 2021, 11:609614. doi: 10.3389/fphys.2020.609614
[5]	Bell S, Gibson JT, Harshfield EL, et al. Is periodontitis a risk factor for ischaemic stroke, coronary artery disease and subclinical atherosclerosis? A Mendelian randomization study[J]. Atherosclerosis, 2020, 313:111-117. doi: 10.1016/j.atherosclerosis.2020.09.029 pmid: 33038664
[6]	郭媛媛, 任佳宇, 冯永亮, 等. 2型糖尿病患者缺牙数目与颈动脉粥样硬化程度的相关性[J]. 口腔医学, 2023, 43(9):791-795.
[7]	唐路, 薛栋, 白雨豪, 等. 颈动脉粥样硬化斑块中牙龈卟啉单胞菌菌毛fimA基因型的分布研究[J]. 现代口腔医学杂志, 2017, 31(6):321-325.
[8]	安娜, 欧阳翔英, 韩蔚. 动脉粥样硬化斑块中牙周可疑致病微生物的检测[J]. 北京大学学报(医学版), 2010, 42(1):33-36.
[9]	Stelzel M, Conrads G, Pankuweit S, et al. Detection of Porphyromonas gingivalis DNA in aortic tissue by PCR[J]. J Periodontol, 2002, 73(8):868-870. doi: 10.1902/jop.2002.73.8.868
[10]	唐路, 薛栋, 白雨豪, 等. 牙龈卟啉单胞菌对血管平滑肌细胞钙化作用的研究[J]. 口腔医学研究, 2018, 34(2):130-133. doi: 10.13701/j.cnki.kqyxyj.2018.02.006
[11]	Murphy SJ, Werring DJ. Stroke:Causes and clinical features[J]. Medicine, 2020, 48(9):561-566. doi: 10.1016/j.mpmed.2020.06.002
[12]	Alpert JN. Extracranial carotid artery. Current concepts of diagnosis and management[J]. Tex Heart Inst J, 1991, 18(2):93-97. pmid: 15227489
[13]	Ong CT, Wong YS, Sung SF, et al. Progression of mild to moderate Stenosis in the internal carotid arteries of patients with ischemic stroke[J]. Front Neurol, 2018, 9:1043. doi: 10.3389/fneur.2018.01043
[14]	Beukers NGFM, van der Heijden GJMG, van Wijk AJ, et al. Periodontitis is an independent risk indicator for atherosclerotic cardiovascular diseases among 60 174 participants in a large dental school in the Netherlands[J]. J Epidemiol Community Health, 2017, 71(1):37-42. doi: 10.1136/jech-2015-206745
[15]	Holt SC, Ebersole JL. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: The red complex, a prototype polybacterial pathogenic consortium in periodontitis[J]. Periodontol 2000, 2005, 38:72-122. doi: 10.1111/prd.2005.38.issue-1
[16]	Mohanty R, Asopa SJ, Joseph MD, et al. Red complex:Polymicrobial conglomerate in oral flora:A review[J]. J Family Med Prim Care, 2019, 8(11):3480-3486. doi: 10.4103/jfmpc.jfmpc_759_19 pmid: 31803640
[17]	Chukkapalli SS, Rivera-Kweh MF, Velsko IM, et al. Chronic oral infection with major periodontal bacteria Tannerella forsythia modulates systemic atherosclerosis risk factors and inflammatory markers[J]. Pathog Dis, 2015, 73(3):ftv009.
[18]	Rivera MF, Lee JY, Aneja M, et al. Polymicrobial infection with major periodontal pathogens induced periodontal disease and aortic atherosclerosis in hyperlipidemic ApoE(null) mice[J]. PLoS One, 2013, 8(2):e57178. doi: 10.1371/journal.pone.0057178
[19]	Yang WW, Guo B, Jia WY, et al. Porphyromonas gingivalis-derived outer membrane vesicles promote calcification of vascular smooth muscle cells through ERK1/2-RUNX2[J]. FEBS Open Bio, 2016, 6(12):1310-1319. doi: 10.1002/2211-5463.12151 pmid: 28255538
[20]	Chen TC, Lin CT, Chien SJ, et al. Regulation of calcification in human aortic smooth muscle cells infected with high-glucose-treated Porphyromonas gingivalis[J]. J Cell Physiol, 2018, 233(6):4759-4769. doi: 10.1002/jcp.v233.6
[21]	Park HJ, Kim Y, Kim MK, et al. Infection of Porphyromonas gingivalis increases phosphate-induced calcification of vascular smooth muscle cells[J]. Cells, 2020, 9(12):2694. doi: 10.3390/cells9122694
[22]	Kim HJ, Cha GS, Kim HJ, et al. Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein[J]. J Periodontal Implant Sci, 2018, 48(1):60-68. doi: 10.5051/jpis.2018.48.1.60
[23]	Koren O, Spor A, Felin J, et al. Human oral, gut, and plaque microbiota in patients with atherosclerosis[J]. Proc Natl Acad Sci USA, 2011, 108(Suppl 1):4592-4598. doi: 10.1073/pnas.1011383107
[24]	Isoshima D, Yamashiro K, Matsunaga K, et al. Microbiome composition comparison in oral and atherosclerotic plaque from patients with and without periodontitis[J]. Odontology, 2021, 109(1):239-249. doi: 10.1007/s10266-020-00524-w
[25]	Brun A, Nuzzo A, Prouvost B, et al. Oral microbiota and atherothrombotic carotid plaque vulnerability in periodontitis patients. A cross-sectional study[J]. J Periodontal Res, 2021, 56(2):339-350. doi: 10.1111/jre.12826 pmid: 33368263
[26]	Xie MR, Tang QM, Yu SL, et al. Porphyromonas gingivalis disrupts vascular endothelial homeostasis in a TLR-NF-κB axis dependent manner[J]. Int J Oral Sci, 2020, 12(1):28. doi: 10.1038/s41368-020-00096-z
[27]	Gupta N, Goswami R, Alharbi MO, et al. TRPV4 is a regulator in P. gingivalis lipopolysaccharide-induced exacerbation of macrophage foam cell formation[J]. Physiol Rep, 2019, 7(7):e14069.
[28]	Mei F, Xie MR, Huang XF, et al. Porphyromonas gingivalis and its systemic impact:Current status[J]. Pathogens, 2020, 9(11):944. doi: 10.3390/pathogens9110944
[29]	段胜仲. 牙周微生态与心脑血管疾病研究进展[J]. 口腔医学, 2021, 41(7):577-582.
[30]	Desvarieux M, Demmer RT, Rundek T, et al. Periodontal microbiota and carotid intima-media thickness:The oral infections and vascular disease epidemiology study (INVEST)[J]. Circulation, 2005, 111(5):576-582. doi: 10.1161/01.CIR.0000154582.37101.15 pmid: 15699278
[31]	Haraszthy VI, Zambon JJ, Trevisan M, et al. Identification of periodontal pathogens in atheromatous plaques[J]. J Periodontol, 2000, 71(10):1554-1560. doi: 10.1902/jop.2000.71.10.1554 pmid: 11063387

特异性引物	序列5'—3'	大小/bp
A.actinomycetemcomitansF:AGAGTTTGATCCTGGCTCAG R:CACTTAAAGGTCCGCCTACGTGCC593
T.forsythiaF:GCGTATGTAACCTGCCCGCA R:TGCTTCAGTGTCAGTTATACCT641
T.denticolaF:TAATACCGAATGTGCTCATTTACAT R:CTGCCATATCTCTATGTCATTGCTCTT860
F.nucleatumF:GGCCACAAGGGGACTGAGACA R:TTTAGCCGTCACTTCTTCTGTTGG183
P.gingivalisF:TGTAGATGACTGATGGTGAAAACC R:ACGTCATCCCCACCTTCCTC197

细菌	重度狭窄组	完全闭塞组	总检出
P.gingivalis	12(33.3)	10(71.4)^*	22(44.0)^#
A.actinomycetemcomitans	1(2.8)	0(0)	1(2.0)
F.nucleatum	4(11.1)	2(14.3)	6(12.0)
T.denticola	10(27.8)	9(64.3)^*	19(38.0)^#
T.forsythia	7(19.4)	6(42.9)	13(26.0)^#

细菌	重度狭窄组	完全闭塞组	总检出
P.gingivalis	22(61.1)	12(85.7)	34(68.0)^#
A.actinomycetemcomitans	4(11.1)	1(7.1)	5(10.0)
F.nucleatum	11(30.6)	2(14.3)	13(26.0)
T.denticola	18(50.0)	10(71.4)	28(56.0)^#
T.forsythia	14(38.9)	9(64.3)	23(46.0)^#

Distribution of periodontal pathogens in carotid atherosclerotic plaques with different degrees of carotid artery stenosis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	GUO Yuanyuan, REN Jiayu, FENG Yongliang, REN Xiuyun. Correlation between the number of missing teeth and the degree of carotid atherosclerosis in type 2 diabetes mellitus patients [J]. Stomatology, 2023, 43(9): 791-795.
[2]	TANG Jinxin, TANG Chunbo, SONG Xin, RUI Na, XUE Chang'ao. Establishment of risk prediction model for peri-implantitis after dental implants in patients with periodontitis [J]. Stomatology, 2023, 43(8): 706-710.
[3]	ZHANG Min, MEI Youmin, YUAN Jianfen, SUN Jingwei, XU Jiali, YAN Junyi, LI Qiao, ZHOU Yan, SHEN Xiang. Correlation analysis between Helicobacter pylori infection and periodontitis in 127 cases [J]. Stomatology, 2023, 43(8): 702-705.
[4]	CAI Bobo, SI Misi. Research progress of immediate implant placement with fixed full-arch prosthesis in patients with severe periodontitis [J]. Stomatology, 2023, 43(7): 663-666.
[5]	LI Shaohua, LIN Xiaoping. Research progress of the relationship between periodontitis and non-alcoholic fatty liver disease [J]. Stomatology, 2023, 43(7): 652-656.
[6]	JIANG Lishan, YANG Zhiyu, SHI Jiahong, WANG Jinmeng, LEI Lang, LI Houxuan. Therapeutic effect and influencing factors of hopeless teeth with Stage Ⅳ Grade C periodontitis: A retrospective study [J]. Stomatology, 2023, 43(7): 633-637.
[7]	ZHANG Yifei, YANG Qi, LI Cheng, LI Lu. Clinical application of Nd:YAG laser to improve the periodontal microecological imbalance in periodontitis patients with type 2 diabetes mellitus [J]. Stomatology, 2023, 43(7): 619-624.
[8]	FENG Zehua, QIU Shuang, XU Xuanwen, ZHENG Kai, XU Yan. In vitro anti-inflammatory effects of mesoporous bioactive glasses/polycaprolactone fibrous bone tissue engineering scaffolds [J]. Stomatology, 2023, 43(7): 592-599.
[9]	TIAN Shilin, CHEN Xiaotao. Research progress of oxidative stress level and iron metabolism in periodontitis [J]. Stomatology, 2023, 43(6): 552-555.
[10]	ZHOU Huirong, LIN Xiaoping. Research advances in correlation between chronic periodontitis and prostatic diseases [J]. Stomatology, 2023, 43(3): 273-277.
[11]	WANG Dexu, LUO Xiaojie, CHEN Xiaotao. Research progress of research on the effect of quorum-sensing system on periodontal pathogens [J]. Stomatology, 2023, 43(3): 267-272.
[12]	XIE Yongmei, GE Huabing, FENG Jie, LIU Qi, ZHANG Ning, ZHONG Xiaoli. A short-term efficacy evaluation of biological antibacterial peptides in the adjuvant therapy of stageⅢ periodontitis [J]. Stomatology, 2023, 43(3): 237-241.
[13]	ZHANG Chaoying, GONG Jiaxing, YU Mengfei, QIAN Ying, ZHU Ziyu, LU Kejie, WANG Huiming. Application progress of alveolar ridge preservation in patients with tooth extracted due to periodontitis [J]. Stomatology, 2023, 43(2): 159-165.
[14]	LEI Gang, WEI Xin, YAN Ming, ZHOU Lili, YU Jinhua, WANG Juan, WU Jintao. Clinical effects of iRoot BP plus and mineral trioxide aggregate in treatment of apical periodontitis with open apical permanent teeth [J]. Stomatology, 2023, 43(2): 118-124.
[15]	DAI Anna,DING Peihui. Periodontal therapy for stage Ⅳ grade C generalized periodontitis: A case report of 7-year follow-up [J]. Stomatology, 2023, 43(2): 145-152.