Abstract: ObjectiveTo investigate the effects of tripterygium wilfordii polyglycosidium (TWP) on the cytotoxicity function of mononuclear-macrophagesin vitro acid environment of squamous-cell carcinoma of tongue.Methods MTT assay was used totest the effects on the cytotoxicity of THP-1 monocytes which were interfered by different concentrations of TWP, and co-cultured with Tca8113 cells in vitro acid environments (pH6.6 or 6.8). Results MTT assay showed thatinterfered by different concentrations of TWP and co-cultured with Tca8113 cells for 4h in the identical vitro acidenvironment(pH6.6 or 6.8), the cytotoxicity of THP-1 monocytes increased as the concentration of TWP increased, and was significantly positively related to the concentration of TWP (P＜0.05). The cytotoxicity also increased with time (p＜0.05) at pH6.6 and TWP of 5×10-1μg/ml, which was significantly positively related to time (P＜0.05). Conclusion Cultured in vitro acid environment of squamous-cell carcinoma of tongue, The cytotoxicity function of mononuclear-macrophages interfered by TWP of certain concentrationincreased compared to those with no interference, and presented significantly positive correlations withTWP concentrations and time.

Key words: acid environment, mononuclear-macrophage, tripterygium wilfordii polyglycosidium（TWP）, squamous-cell carcinoma of tongue