氯通道阻断剂NPPB对鸡喙外胚间充质细胞增殖分化影响的研究

Abstract

Abstract: Objective To study the effect of Chloride channel blocker NPPB on the proliferation and differentiation of chicken mandibular mesenchymal cells (CMMC). Methods In the study, chicken mandibular mesenchymal cells (CMMC) were isolated and chondrocyte maturation was induced by ascorbic acid and b-glycerophosphate (AA-BGP). The effect of the chloride channel inhibitor NPPB [5-nitro-2-(3-phenylpropylamino)benzoic acid] on regulation of growth, and changes in Col10、Sox9 and Runx2mRNA genes expressions were observed with MTT and real-time PCR assays. Results NPPB inhibited the growth of the CMMC with/without AA-BGP treatment(P< 0.05). NPPB antagonized expression of typeX collagen and Runx2 (P < 0.05) with/ without AA-BGP treatment, whilst it had no effect on the early chondrocyte differentiation marker Sox9. Conclusion We conclude that chloride channel in CMMC plays an important role in regulating chondrocyte proliferation and plays a major role in the progression of chondrocyte differentiation into hypertrophic cartilage. The data from the current study suggest that maybe chloride channel works in chondrocyte differentiation by regulating the Runx2 level.

Key words: chloride channel, NPPB, chicken mandibular mesenchymal cells, cell proliferation and differentiation

CLC Number:

R349.5

References

[1]Jentsch TJ.Chloride and the endosomal–lysosomalpathway: emerging roles of CLC chloride transporters[J].Physiol,2007,578(Pt 3):633-640
[2]Jentsch TJ.CLC chloride channels and transporters: from genes to protein structure,pathology and physiology[J].Crit Rev BiochemMolBiol,2008,43(1):33-36
[3]Hou J,Situ Z,Duan X.ClC chloride channels in tooth germ and odontoblast-like MDPC-23cells[J].Arch Oral Biol,2008,53(9):874-878
[4]Yin Z,Tong Y,Zhu H,et al.ClC-3 is required for LPA- activated Cl-current activity and fibroblast-to- myofibroblastdifferentiation[J].Am J Physiol Cell Physiol,2008,294(2):C535-542
[5]Kornak U,Kasper D,Bosl MR,et al.Loss of the ClC-7chloridechannel leads to osteopetrosis in mice and man[J].Cell,2001,104(2):205-215
[6]Wang SS,Devuyst O,Courtoy PJ,et al.Mice lack ingrenal chloride channel,CLC-5,area model for Dent’s disease,a nephrolithiasis disorder associated with defective receptor-mediated endocytosis[J].Hum Mol Genet,2000,9(20):2937-2945
[7]Matsuda JJ,Filali MS,Volk KA,et al.OverexpressionofCLC-3inHEK293Tcellsyields novel currents that are pH dependent[J].Am J Physiol Cell Physiol,2008,294(1):C251-262
[8]侯晋，段小红,司徒镇强.阻断氯离子通道对小鼠成牙本质细胞MDPC-23生物学特性影响的研究[J].牙体牙髓牙周病学杂志,2007,17(6):316-319
[9]Kotake S,Yago T,Kawamoto M,et al.Voltage-dependent anion channels (VDACs,porin) expressed in the plasma membrane regulate the differentiation and function of human osteoclasts[J].CellBiol Int,2013,37(1):65-77
[10]Ekanayake S,Hall BK.Hypertrophy is not a prerequisite for type X collagen expression or mineralization of chondrocytes derived from cultured chick mandibular ectomesenchyme[J].Int J DevBiol,1994,38(4):683-694
[11]Bi W,Huang W,Whitworth DJ,et al.Haploinsufficiency of Sox9 results in defective cartilage primordia and premature skeletal mineralization[J].Proc Natl Acad Sci USA,2001,98(12):6698-6703
[12]Terry A,Kilbey A,VaillantF,et al.Conservation and expression of an alternative 3' exon of Runx2 encoding a novel proline-rich C-terminal domain[J].Gene,2004,336(1):115-125
[13]杨翔云，赖小刚，张勇,等.干扰ClC基因表达对胶质瘤BT-325细胞生长的影响[J].中国康复理论与实践,2006,12(3):378-380
[14]Li M,Wang B,Lin W.Cl-channel blockers inhibit cell proliferation and arrest the cell cycle of human ovarian cancer cells[J].Eur J GynaecolOncol,2008,29(3):267-271
[15]Duan X,Mao Y,Yang T,et al.ClC-5 regulates dentin development through TGF-beta1 pathway[J].Arch Oral Biol,2009,54(12):1118-1124
[16]Takeda S,Bonnamy JP,Owen MJ,et al.Continuous expression of Cbfa1 in nonhypertrophic chondrocytes uncovers its ability to induce hypertrophic chondrocyte differentiation and partially rescues Cbfa1-deficient mice[J].Genes Dev,2001,15(4):467-481

Study on effect of Chloride channel blocker NPPB on the proliferation and differentiation of chicken mandibular mesenchymal cells

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