咬合改变和束缚应激中小鼠全脑神经激活的比较研究

doi:10.13591/j.cnki.kqyx.2022.06.001

Abstract

Abstract: Objective To observe the activation of neurons in the whole brain caused by occlusal changes in mice, and compare it with that in restraint stress in order to explore the neural mechanism of anxiety induced by occlusal changes in mice. Methods Adult C57BL/6J male mice were randomly selected for the experiment. On the 7th and 8th days after occlusal elevation, behavior test were performed after the surgery by analyzing movement in the open field and elevated plusmaze. The expression of cfos protein in the whole brain was detected by immunofluorescence in 2 hours after the surgery and the acute restraint stimuli. Results It was found that anxiety-like behaviors in OVR group increased significantly a week after surgery (P＜0.05), and the number of cfos-positive neurons in claustrum, nucleus accumbens, lateral septal nucleus, paraventricular hypothalamic nucleus and the nucleus of the solitary tract increased significantly in both stimuli (P＜0.05). Conclusion There are differences in the activation of neurons in the whole brain of mice after occlusal change or restraint, but they are co-activated in the claustrum, nucleus accumbens, lateral septal nucleus, paraventricular hypothalamic nucleus and the nucleus of the solitary tract.

Key words: occlusal change, anxiety, restraint, mice

CLC Number:

R780.1

XU Ming, LI Juan, LIU Weicai, ZHANG Qi. Comparison of the activation of whole-brain neurons between occlusal change and restraint[J]. Stomatology, 2022, 42(6): 481-486.

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Comparison of the activation of whole-brain neurons between occlusal change and restraint

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