新型镁合金黏膜刺激性、急性全身毒性及抗菌性能研究

doi:10.13591/j.cnki.kqyx.2023.10.003

Abstract

Abstract:

Objective To determinethe mucosal irritant, acute systemic toxicity and antibacterial propertiesof novel magnesium alloys(Mg-3Sn-0.5Mn). Methods Through the mucosal stimulation experiment of golden gopher and the acute toxicity experiment of mice, the biocompatibility of the new magnesium alloy was tested. The bacterial state on the surface of the new magnesium alloy was observed by plate counting and scanning electron microscopy after the culture of magnesium alloy and bacterial liquid(Escherichia coli and Staphylococcus aureus) for 1 d and 3 d. Results Acute systemic toxicity experiment of mice showed that during the 72 h observation period, there was no acute toxicity reaction in the experimental group and the mice in the control group. Mucosal stimulation experiment showed that after 14 d, there was no obvious abnormality between the experimental group and the control group in the cheek mucosa HE staining and gross observation of the golden gopher. Antibacterial experiment revealed that compared with 316 stainless steel in the control group, the number of bacterial colonies on the surface of the petri dish was significantly smaller, and the antibacterial effect was obvious. According to scanning electron microscope observation,the number of Staphylococcus aureus on the surface of magnesium alloy was significantly less than that of the control group, and the shape was obviously wrinkled. Conclusion The new magnesium alloy(Mg-3Sn-0.5Mn)has good biocompatibility and antibacterial properties, which can initially meet the basic requirements of medical materials.

Key words: magnesium alloy, biocompatibility, antibacterial property, mucosal stimulation experiment

CLC Number:

R783.1

CHU Maoran, LIU Juan, XIU Zhaobo, SUN Yu. Study on mucosal irritation, acute systemic toxicity and antibacterial properties of the novel magnesium alloys[J]. Stomatology, 2023, 43(10): 878-882.

Figures/Tables 7

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References 20

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分组	实验前	24 h	48 h	72 h
Mg-3Sn-0.5Mn	24.95±2.21	25.68±2.24	27.08±2.26	28.32±2.33
对照组	25.70±2.45	27.19±3.00	29.01±3.03	30.09±3.27

菌种	分组	菌落中位数/个		Z	P
菌种	分组	实验组	对照组	Z	P
S.aureus	1 d游离菌	601	996	-2.402	0.016
	1 d黏附菌	437	980	-2.402	0.016
	3 d游离菌	241	1 389	-2.611	0.009
	3 d黏附菌	141	508	-2.611	0.009
E.coli	1 d游离菌	119	445	-2.611	0.009
	1 d黏附菌	138	903	-2.611	0.009
	3 d游离菌	9	2 048	-2.611	0.009
	3 d黏附菌	11	1 460	-2.611	0.009

Study on mucosal irritation, acute systemic toxicity and antibacterial properties of the novel magnesium alloys

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