hSSB1在口腔鳞状细胞癌中的表达及临床意义

doi:10.13591/j.cnki.kqyx.2026.02.007

Abstract

Abstract:

Objective To investigate the expression and clinical significance of human single-stranded DNA-binding protein 1 (hSSB1) in oral squamous cell carcinoma(OSCC). Methods A total of 30 OSCC patients treated at the Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Guilin Medical University between January 2022 and December 2023 were included. The expression levels of hSSB1 in OSCC tissues and adjacent normal tissues were detected using immunohistochemistry (IHC), Western blot, and RT-qPCR. The correlation between hSSB1 expression and clinicopathological features was analyzed. Results Both protein and mRNA expression levels of hSSB1 were significantly elevated in OSCC tissues compared with adjacent normal tissues (P<0.05). While hSSB1 mRNA expression was not significantly associated with any clinicopathological parameters (P>0.05), hSSB1 protein expression showed significant correlations with age, lymph node metastasis, and clinical stage (P<0.05). Conclusion hSSB1 is significantly up-regulated in OSCC and may play a role in the development and progression of the disease.

Key words: hSSB1, repair of DNA damage, oral squamous cell carcinoma, pathogenesis

CLC Number:

R739.86

ZENG Xianglin, LIU Jimu, QIN Xiaorong, LI Bo. Expression and clinical significance of hSSB1 in oral squamous cell carcinoma[J]. Stomatology, 2026, 46(2): 126-130.

Figures/Tables 5

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References 26

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基因	引物序列	扩增长度/bp
hSSB1	上游引物 5'-ACATTATCCGGCTCACCAAAG-3'	135
hSSB1	下游引物 5'-GCTCACTGAAGTTAGGAACCTCA-3'	135
β-actin	上游引物 5'-CATGTACGTTGCTATCCAGGC-3'	250
β-actin	下游引物 5'-CTCCTTAATGTCACGCACGAT-3'	250

临床参数		n	mRNA	统计量	P	蛋白	统计量	P
性别	男	26	2.99±1.32	t=-0.088	0.930	0.89±0.08	t=-0.128	0.899
性别	女	4	3.05±1.84	t=-0.088	0.930	0.89±0.05	t=-0.128	0.899
年龄/岁	≤55	15	3.20±1.34	r=-0.082	0.666	0.91±0.06	r=-0.392	0.032
年龄/岁	>55	15	2.78±1.40	r=-0.082	0.666	0.87±0.08	r=-0.392	0.032
直径/cm	≤2	13	3.33±1.43	r=-0.243	0.195	0.89±0.07	r=-0.392	0.518
直径/cm	>2	17	2.74±1.29	r=-0.243	0.195	0.89±0.08	r=-0.392	0.518
病理分级	高分化	21	3.05±1.30	H=0.498	0.780	0.90±0.06	H=3.672	0.159
	高-中分化	5	3.11±1.46			0.91±0.11
	中-低分化	4	2.54±1.84			0.84±0.08
临床分期	Ⅰ	11	3.21±1.51	H=0.307	0.959	0.88±0.07	H=11.948	0.008
	Ⅱ	9	2.90±1.35			0.94±0.06
	Ⅲ	5	2.94±1.10			0.91±0.05
	Ⅳ	5	2.74±1.63			0.81±0.02
淋巴结转移	无	21	3.09±1.38	t=-0.596	0.556	0.91±0.07	t=-2.385	0.024
淋巴结转移	是	9	2.78±1.38	t=-0.596	0.556	0.85±0.06	t=-2.385	0.024

Expression and clinical significance of hSSB1 in oral squamous cell carcinoma

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