PTK7在口腔鳞癌中的表达分析及其生物学功能研究

doi:10.13591/j.cnki.kqyx.2024.04.006

Abstract

Abstract:

Objective To explore the expression level of PTK7 in OSCC, and study its potential biological function and clinical significance. Methods qPCR and Western blot were performed to detect the expression of PTK7 in OSCC cell lines and OSCC tissue specimens. siRNA interference technology was used to down-regulate the expression of PTK7 in HN6 and Cal27 cell lines. The effects of down-regulated PTK7 on the proliferation, migration, invasion of OSCC cells were detected by CCK-8 assay, plate cloning assay, cell scratch assay, Transwell assay. Immunohistochemistry was used to detect the expression level of PTK7 protein in 75 cases of OSCC tissue. Results The results of qPCR and Western blot showed that PTK7 gene and its coding protein were highly expressed in OSCC lines HN6 and Cal27, and the expression of PTK7 gene and its coding protein was higher in 6 fresh OSCC samples than in their paired adjacent normal tissues. Down-regulating the expression of PTK7 could inhibit the proliferation, migration and invasion of OSCC cells. In the 75 OSCC specimens, the expression level of PTK7 was significantly associated with the patients’ age, smoking and differentiation degree (P<0.05). Kaplan Meier survival analysis found that OSCC patients with high expression of PTK7 had poorer prognosis. Conclusion PTK7 is a potential oncogene in the occurrence and development of OSCC, and its expression level affects the biological function of OSCC cells. The characteristics of OSCC can be understood clinically according to the expression level of PTK7, and PTK7 can be used as an independent prognostic indicator for OSCC.

Key words: oral squamous cell carcinoma, protein tyrosine kinase 7, proliferation, migration, invasion

CLC Number:

R782
R739.8

SUN Xinyi, PAN Yuetong, LU Xinyue, LYU Zhongjing, YUAN Jian, LI Jiafeng, SHI Huan. Analysis of PTK7 expression in oral squamous cell carcinoma and its biological function[J]. Stomatology, 2024, 44(4): 268-275.

Figures/Tables 11

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References 24

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特征	样本含量n(%)	PTK7表达量	P
性别			0.052
男	45(60)	2.282±0.708
女	30(40)	1.947±0.743
年龄/岁			0.005
<60	27(36)	1.832±0.720
≥60	48(64)	2.326±0.691
吸烟			0.003
是	24(32)	2.504±0.659
否	51(68)	1.981±0.717
饮酒			0.628
是	24(32)	2.088±0.814
否	51(68)	2.177±0.704
淋巴结转移			0.590
是	21(28)	2.222±0.680
否	54(72)	2.119±0.762
分化程度			0.000
低分化	27(36)	2.563±0.624
高分化	48(64)	1.915±0.696

预后因素	样本含量n(%)	HR	95%CI	P
性别		1.351	0.599~3.264	0.503
男	45(60)
女	30(40)
年龄/岁		1.925	0.794~4.667	0.148
<60	27(36)
≥60	48(64)
吸烟		3.538	1.156~10.830	0.027
是	24(32)
否	51(68)
饮酒		0.385	0.144~1.034	0.058
是	24(32)
否	51(68)
淋巴结转移		4.076	1.565~10.619	0.004
是	21(28)
否	54(72)
分化程度		1.636	0.777~3.446	0.195
低分化	27(36)
高分化	48(64)
PTK7表达		0.331	0.156~0.702	0.004
低表达	37(49.3)
高表达	38(50.7)

Analysis of PTK7 expression in oral squamous cell carcinoma and its biological function

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