口腔鳞癌环状RNA差异表达谱分析及circ_PVT1致癌机制的研究

doi:10.13591/j.cnki.kqyx.2025.09.003

Abstract

Abstract:

Objective To screen the differential expression profile of circ_RNA in OSCC and to elucidate the molecular mechanism of circ_PVT1 on OSCC carcinogenesis. Methods The transcripts of 3 cases of OSCC and normal tissues were sequenced by high-throughput sequencing using circ_RNA expression profile chip, and the differential gene expression profiles were screened, and GO and KEGG enrichment analysis were performed. The expression level of PVT1 in OSCC tissues, human normal oral mucosal cells and OSCC cells was detected by qRT-PCR. The effect of PVT1 on the biological behavior of SCC-25 and SCC-9 cells was evaluated by MTT experiment, Transwell experiment and flow cytometry. The effect of PVT1 on the expression of key proteins in the Wnt3a/β-catenin pathway was evaluated by Western blot. The relationship between the expression of PVT1 and clinical pathological characteristics and prognosis of patients was further studied. Results A total of 403 differentially expressed circ_RNAs were screened by the chip, and the differentially expressed genes were enriched in pathways related to cancer progression. PVT1 was highly expressed in OSCC tissues and cells. Silencing PVT1 expression could inhibit the activation of the Wnt3a/β-catenin pathway, thereby effectively inhibiting the proliferation, migration, invasion and cell cycle of SCC-25 and SCC-9 cells and promoting apoptosis. PVT1 expression was only associated with lymph node metastasis and distant metastasis in patients, and those with high expression had a shorter PFS. Conclusion PVT1 promotes the progression of OSCC by regulating the activation of Wnt3a/β-catenin pathway. The research results provide new ideas for the diagnosis and treatment of OSCC.

Key words: oral squamous cell carcinoma, gene chip, circ_PVT1, Wnt3a/β-catenin, pathway

CLC Number:

R739.85

WU Liwei, QIU Yongle, ZHAO Jiahong, ZHUANG Zhizheng, LI Kunshan. Analysis of differential expression profiles of circular RNA in oral squamous cell carcinoma and study on the carcinogenic mechanism of circ_PVT1[J]. Stomatology, 2025, 45(9): 655-662.

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References 23

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siRNAs	上游引物	下游引物
PVT1-siRNA1	5'-GGCUUGAGGCCUGAUCUUUTT-3'	5'-AAAGAUCAGGCCUCAAGCCTT-3'
PVT1-siRNA2	5'-GGCUUGAGGCCUGAUCUUUTT-3'	5'-AAAGAUCAGGCCUCAAGCCTT-3'
PVT1-siRNA3	5'-GCUGGGCUUGAGGCCUGAUTT-3'	5'-AUCAGGCCUCAAGCCCAGCTT-3'
NC-siRNA	5'-GCUACGAUCUGCCUAAGAUTT-3'	5'-AUCUUAGGCAGAUCGUCGCTT-3'

circRNAs	Fold-change	P	Hostgene
上调
hsa_circ_0090990	33.273 63	0.000 58	ZMYM3
hsa_circ_0000423	32.632 89	0.002 32	PPP1R12A
hsa_circ_0036540	25.440 78	0.008 44	MESDC2
hsa_circ_0078337	21.192 85	0.005 08	IPCEF1
hsa_circ_0030556	12.168 26	0.007 03	MIR17HG
hsa_circ_0013128	7.115 96	0.008 25	CDC7
hsa_circ_0054291	6.853 83	0.004 89	ZFP36L2
hsa_circ_0070429	6.847 98	0.004 47	HERC3
hsa_circ_0009077	5.820 61	0.002 65	TYW1B
hsa_circ_0033689	5.770 88	0.000 25	ADAM6
下调
hsa_circ_0058498	43.273 63	0.008 62	COL4A4
hsa_circ_0008269	32.632 89	0.002 49	USP8
hsa_circ_0056924	22.440 78	0.007 76	SCN9A
hsa_circ_0008304	15.192 85	0.008 66	TFDP2
hsa_circ_0001323	10.168 26	0.001 16	BBX
hsa_circ_0038262	10.115 96	0.000 86	SMG1
hsa_circ_0068421	8.853 83	0.009 88	TRA2B
hsa_circ_0073527	6.847 98	0.009 35	FER
hsa_circ_0035196	5.820 61	0.002 36	ATP8B4
hsa_circ_0031817	5.770 88	0.009 87	METTL21D

临床病理特征分类	n	PVT1		χ²	P
临床病理特征分类	n	高表达 (27)	低表达 (27)	χ²	P
年龄
<55岁	19	11	8	0.393	0.731
≥55岁	35	16	19
性别
男	25	15	10	1.862	0.172
女	29	12	17
吸烟
有	32	18	14	1.227	0.268
无	22	9	13
肿瘤分期
T1、T2	33	16	17	0.078	0.780
T3、T4	21	11	10
淋巴结转移
有	16	12	4	5.684	0.017
无	38	15	23
远处转移
有	24	8	16	4.800	0.028
无	30	19	11

Analysis of differential expression profiles of circular RNA in oral squamous cell carcinoma and study on the carcinogenic mechanism of circ_PVT1

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