抑制MIF活性对大鼠种植体周围炎骨吸收的影响

doi:10.13591/j.cnki.kqyx.2026.05.005

Abstract

Abstract:

Objective To investigate the effect of inhibiting macrophage migration inhibitory factor（MIF） activity on bone resorption in rat peri-implantitis. Methods Twenty male Sprague-Dawley（SD） rats were randomly divided into four groups： blank control group，inflammation control group，inflammation treatment group，and inflammation solvent group. After extracting the bilateral first molars of each rat，titanium alloy cortical bone screws were immediately implanted. In all inflammation groups，peri-implantitis models were constructed by ligating the implants with silk thread. Subsequently，the peri-implant gingiva of rats in the blank control group and inflammation control group was injected with normal saline； the inflammation treatment group was injected with MIF inhibition ISO-1，and the inflammation solvent group was injected with dimethyl sulfoxide（DMSO） solution. Micro-CT scanning was used to observe the peri-implant bone resorption，and analyze bone mineral density（BMD） and bone volume fraction（BV/TV）. HE staining was performed to observe the histological manifestations of peri-implant tissues. Immunohistochemical analysis was used to detect the protein expression levels of IL-6 and IL-10. Results Compared with the blank control group，the BMD and BV/TV in the three inflammation groups were significantly decreased（P<0.05）； HE staining showed inflammatory cell infiltration and fibrous connective tissue formation； the expression levels of IL-6 and IL-10 were increased（P<0.05）. After local inhibition of MIF activity，compared with the inflammation control group and inflammation solvent group，the inflammation treatment group showed increased BMD and BV/TV，decreased IL-6 expression，and increased IL-10 expression（P<0.05）. Conclusion Inhibition of MIF activity can alleviate bone loss in peri-implantitis，and the mechanism may be related to the decrease in IL-6 expression and the increase in IL-10 expression.

Key words: peri-implantitis, macrophage migration inhibitory factor, ISO-1, IL-6, IL-10

CLC Number:

R782.13

GAO Jingzhe, CAO Jiajun, LI Haixu, SUN Yu. The effect of inhibiting MIF activity on bone resorption in rat peri-implantitis[J]. Stomatology, 2026, 46(5): 350-355.

Figures/Tables 8

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References 28

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组别	BV/TV	BMD/（g/cm³）
空白对照组	0.82±0.06^#	1.10±0.07^#
炎症对照组	0.46±0.12^*#	0.46±0.11^*#
炎症治疗组	0.64±0.17^*	0.82±0.11^*
炎症溶剂组	0.45±0.20^*#	0.62±0.16^*#

组别	IL-6/%	IL-10/%
空白对照组	1.52±0.96^#	3.39±1.68^#
炎症对照组	8.47±3.05^*#	8.95±1.83^*#
炎症治疗组	5.13±1.01^*	14.51±6.39^*
炎症溶剂组	8.88±3.26^*#	8.97±3.28^*#

The effect of inhibiting MIF activity on bone resorption in rat peri-implantitis

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