负载锶离子水凝胶促进人牙周膜干细胞成骨分化的转录组学特征及分子网络初探

doi:10.13591/j.cnki.kqyx.2026.05.006

Abstract

Abstract:

Objective To investigate the effect of an injectable photo-crosslinking hydrogel loaded with strontium ions （PLSr²⁺@HAMA） on osteogenic differentiation-related phenotypes of human periodontal ligament stem cells （hPDLSCs） and to analyze its potential molecular regulatory features through transcriptomic analysis. Methods Cell viability of hPDLSCs was evaluated using the CCK-8 assay. Alizarin Red S （ARS） staining，real-time quantitative PCR （RT-qPCR），and Western blot （WB） were used to detect changes in osteogenic-related markers. RNA-seq was performed，and bioinformatic analysis tools were used to conduct functional enrichment and protein-protein interaction network analysis of differentially expressed genes. Results PLSr²⁺@HAMA hydrogel did not significantly affect cell proliferation. ARS staining showed enhanced mineralization nodule formation. RT-qPCR and WB results indicated increased expression of alkaline phosphatase （ALP） and bone morphogenetic protein 2 （BMP2）. Transcriptomic analysis revealed that differentially expressed genes were mainly associated with extracellular matrix regulation，signal transduction，and cellular responses. Several hub genes related to osteogenesis and matrix regulation were identified. Conclusion PLSr²⁺@HAMA hydrogel promotes osteogenic differentiation of hPDLSCs and induces changes in osteogenic-related transcriptional responses，providing experimental support for its application in periodontal tissue regeneration.

Key words: hydrogels, osteogenesis, transcriptomics, human periodontal ligament stem cells, strontium ions

CLC Number:

R781.4

DAI Jiayun, WANG Xingyang, ZHOU Duo, LU Yiyao, WANG Juan. Strontium ion-loaded hydrogel promotes osteogenic differentiation of hPDLSCs： Transcriptomics and molecular network analysis[J]. Stomatology, 2026, 46(5): 356-362.

Figures/Tables 6

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References 31

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基因名称	引物序列（5'→3'）
BMP2	F：AAGCCAAACACAAACAGCGG R：ATCAGCCAGAGGAAAAGGGC
ALP	F：CTTGCTGGTGGAAGGAGGCAGG R：CACGTCTTCTCCACCGTGGGTC
GAPDH	F：GATGGTGAAGGTCGGTGTG R：GAGGTCAATGAAGGGGTCG

Strontium ion-loaded hydrogel promotes osteogenic differentiation of hPDLSCs： Transcriptomics and molecular network analysis

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