Objective To study the effect of cryopreservation on the dedifferentiation of the condylar cartilage cells. Methods Condylar cartilage was aseptically dissected from the temporomandibular joint of 2 week old New Zealand white rabbit. The condylar cartilage cells were subcultured and analyzed after culturing in vitro to the 1st passage and then frozen for 15 days as the experimental group. The cytoactive of the 2nd passage cells of the experiment group and that of the control group were assessed by inverted phase contrast microscope. Quantitative realtime PCR and Western blot technique were used to analyze the expression of Collagen Type Ⅱ, Collagen Type Ⅹ, Sox9 and Aggrecan. One-way ANOVA was performed using SPSS13.0 software package. Results The 2nd passage of the experimental group did have morphological changes, but Quantitative realtime PCR and Western blot both showed that there was no significant difference in the expression of Collagen Type Ⅱ,Collagen Type Ⅹ, Sox9 and Aggrecan between the experiment group and the control group. Both the experiment group and the control group would have a lower expression of those cartilage specific genes than the 1st passage cells. Conclusions Cell cryopreservation will not accelerate the dedifferentiation progress of condylar cartilage cells during subcultivation. That means the condylar cartilage cells cultured in vitro can be stored by cryopreservation.

Objective To explore the preparation of several kinds of morphologies of ZnO HAPw with different temperatures and pH values.Methods Hydroxyapatite whisker was modified by Zinc acetate via solgel,which was stirred,concussed,spun and annealed,and then white powder was obtained.At last,it was analyzed by transmission electron microscopy scanning electron microscope and energy dispersive Xray spectroscopy.Results Four kinds of morphologies of ZnOHAPw were granule,triangle,short rod and gongs nail at pH=6.4 and 70 ℃,pH=6.6 and 70 ℃,pH=6.6 and 37 ℃,pH=6.6 and 37 ℃ respectively.It was demonstrated that the substance on the whisker surface was ZnO by analyzing energy dispersive Xray spectroscopy.Conclusions Hydroxyapatite whisker could be modified by Zinc acetate via solgel.And different morphologies of ZnOHAPw can be produced with different temperatures and pH values.

Objective To investigate the therapeutic effect of Huashi Xingyu Qingre decoction on oral lichen planus.Methods 30 cases of OLP patients and 30 normal people were selected and the content of ALT,AST,LDH in saliva was detected by automatic biochemical analyzer,the OLP patients were treated by Huashi Xingyu Qingre decoction,and the clinical effect 6 weeks after treatment and the changes of ALT,AST,LDH in saliva before and after treatment were observed.Results In the group of patients with OLP by Huashi Xingyu Qingre decoction 6 weeks after treatment,symptoms disappeared or reduced to different degrees,and hyperemia,erosion,white lesions disappeared or reduced; in the group of patients with OLP,its LDH content in saliva was significantly higher than that of the normal control group.There was no obvious difference between OLP group and the control group in ALT,AST content,but the content of LDH by Huashi Xingyu Qingre decoction reduced obviously in the saliva 6 weeks after treatment.Conclusions Huashi Xingyu Qingre decoction has obvious clinical effect on oral lichen planus; Salivary ALT,AST levels had no significant relationship with OLP,and the increased LDH levels may be associated with the occurrence of OLP.

Objective To evaluate the clinical effect of preoperation induction chemotherapy with TPF for advanced oral squamous cell carcinoma.Methods From June 2008 to June 2010,48 patients with advanced oral squamous cell carcinoma received induction chemotherapy (D1 Docetaxel 75 mg/m2,D1 Cisplatin 75 mg/m2,D1D5 5fluorouracil 750 mg/m2).Two week after two cycles of chemotherapy,the effects before and after chemotherapy were compared based on the tumor volume and the effects.All cases were divided into complete response (CR),partial response(PR),stable disease(SD) and progressive disease(PD),and CR+PR were effective cases.Results The overall response rate was 58.33%（28/48）with 2 cases of CR，26 cases of PR，18 cases of SD and 2 cases of PD,with no serious adverse reactions.Conclusions Induction chemotherapy with TPF can decrease the tumor volume of advanced oral squamous cell carcinoma significantly.The shortterm effects of this method are marked,while further observation will be needed for the evaluation of longterm effects.

Objective To study the possibility of using bFGFcollagen slow release system for repairing bone defect.Methods 60 Japanese white rabbits were randomly divided into three groups: the bFGF collagen sponge group,the collagen sponge group and the control group.Then the bone defects were made in the rabbits′ bilateral mandibles,and the biological materials corresponding with each group were inserted into defects.15 of animals were sacrificed at 2、6、8、12 weeks to be evaluated with anatomy,CT studies and histology.Results General observation and CT imaging showed a faster velocity of bone formation in the control groups.Histological observation showed that the effect of bone repairing in the experimental group was much better than that in the control group.The collagen in vivo lasted until 6 weeks ago,and the bFGF which was continuously released from it had obvious promoting effect on bone defect repair,while the action time was mainly 12 weeks ago.Conclusions The bFGFcollagen slow release system has great promoting effect on repairing rabbit mandibular defect,while the action time is mainly in the early stage of bone defect repairing.Besides,the collagen sponge itself also has promoting effect on mandibular defect repair.

Objective To carry out independent research with the method of selfdesign CAD/CAM for implant guide.Methods The software design and development of CAD/CAM dental implant guide were completed together with NUAA (Nanjing University of Aeronautics and Astronautics),and combining with the CBCT(Conebeam computed tomography) data of the jawbone and the prostheses to complete modeling,rectification and designing of the implant guide .Results The CT data were successfully imported into the software,the 3D reconstruction was completed,and rectification of the jawbone and the prostheses have been achieved and the implant guide has been successfully designed.Conclusions The designing software of the CAD/CAM implant guide has been successfully developed,which is of great clinical application value.

Objective To evaluate the clinical effects and strategy of periodontal therapy on different margin levels of residual tooth to analyze the indications of residual roots reserved．Methods 187 residual roots in 85 cases were included in the study．They were divided into three groups according to PD value,margin level of tooth structure to bone (MTB),and different periodontal therapeutic strategies: (1) Group therapy 1 (T1): 67 residual roots with PD≥3 mm,MTB≥4 mm,receiving gingivectomy; (2) Group therapy 2 (T2): 71 residual roots with PD<2 mm and the MTB< 3 mm,receiving crown lengthening surgery; (3) Group control (C): 49 residual roots with PD<2 mm and MTB <3 mm,receiving gingivectomy.SBI,GI and tooth mobility of different groups before operation,1 week,4 weeks,six months and 1 year after operation were measured.Results The success rate of residual roots was 97.3%.There was significant difference in SBI and GI value when comparing the group T1,T2 with C at different observation points.The tooth mobility ratio of group T1 and T2 decreased at 6 months or 1 year after operation comparing with that before therapy,while that of group C increased at 6 months or 1 year after therapy.The success rates of group T1 and T2 were significant higher than group C at 1 week and 1 year after operation.Conclusions Rational periodontal strategy and approach display significant value on different margin levels of residual tooth,which can significantly improve success rate of residual roots reserved.

Objective To develop a new measuring system individually customized for oral cavity as close as possible to the natural physiological state and to explore an approach to assess the distribution of tongue pressure during major oral functions in upper dental arch. Methods The measuring system consists of a piezoresistive sensor sheet with 0.1 mm thickness, a device for data collection and PC software for pressure display. After calibration and test, the sensor sheet was attached to five measuring points on the palatal side of upper dental arch of a female adult volunteer with individual normal occlusion to measure tongue pressure during major oral functions. Feasibility of the new measuring system and method was evaluated. Results A new measuring system as close as possible to the natural physiological state was developed. Measuring method based on this system achieved satisfactory operation consistency. Conclusions The new measuring system and method is feasible and useful for future research with good application prospect.

Objective To discuss the chin soft tissue thickness of adults with different skeletal patterns. Methods All the subjects were divided into three groups according to different skeletal patterns and each group included 20 patients.The images were constructed into 3dimensional (3D) model using medical image processing software Dolphin Imaging 11.0.The reference planes were established and the two points,pogonion of hard tissue (Po) and soft tissue (Pos) were located on the 3D model.The chin soft tissue thickness was calculated statistically. Results There was no gender difference in terms of the chin soft tissue thickness (P>0.05) and it was not affected by skeletal patterns.The coordinate of it was affected by the skeletal patterns (class Ⅰ and class Ⅱ,class Ⅱ and class Ⅲ).There were significant differences in the z coordinate of point Po (P<0.05,class Ⅰ and class Ⅲ; class Ⅱ and class Ⅲ). Conclusions The chin soft tissue thickness of different skeletal patterns showed significant differences and the differences existed in the horizontal plane primarily.There were significant differences in Point Po between class Ⅰ and class Ⅱ,class Ⅱ and class Ⅲ,which would provide guidance for the diagnosis and orthodontic treatment.

Objective 〖JP2〗To discuss the clinical effects and rationality of partial parotidectomy in the treatment of parotid benign tumors.Methods Among 103 cases of parotid benign tumors,53 cases were treated with partial parotidectomy.The ectomy field was extended 0.51.0 cm from the border line of tumor,with conservation of fascia parotideamasseterica and branch(s) of great auricular nerve; the other 50 patients were treated with conventional superficial parotidectomy.All the patients were followed up for 0.55 years.The differences in the incidence of complications and tumor recurrence between the 2 groups were compared by using Chisquare tests.Results The degree of facial deformity was mild,and the function of parotid was fine after partial parotidectomy.The occurrence rates of Frey syndrome,xerostomia,earlobe numb,facial deformity,temporary facioplegia and salivary fistula in the partial parotidectomy group were significantly (P<0.05) lower than the superficial parotidectomy group.Tumor recurrence was not observed in both groups.Conclusions Partial parotidectomy can completely resect the parotid benign tumors,and significantly decrease the occurrence rate of complications.

Objective To evaluate the clinical effect of muscular position splint on anterior disk displacement with reduction,to analyze the result of the treatment,and to investigate the factors that influence the effect of the treatment.Methods 89 patients (23 men and 66 women),who were diagnosed as anterior disk displacement with reduction by clinical and MRI examination participated in this study.All patients received muscular position splint treatment,and the total course of treatment was 3～6 months .We recorded the condition of recovery 1 week,4 weeks and 12 weeks after treatment.When the treatment was finished,the patients were divided into two different groups according to age and medical history,and the date was retrospectively analyzed.Results The patients who were younger than 17 and those whose clicking time was shorter than two weeks showed better outcome than others,and the recovery rates were respectively 88.9% and 90.9%.The results were analyzed by chisquare test,which showed in age group the χ2=41.53(P<0.05) while in history group χ2=23.94(P<0.05),and there were statistically significant differences in two groups.Conclusions Muscular position splint is more effective in the treatment of anterior disk displacement with reduction in younger patients whose clicking time is shorter than two weeks,thus occlusal splint should be recommended at the early stage of the temporomandibular joint clicking for the possibility of this condition to be cured as we expect.

Objective To seek a safe and convenient anesthesia method for the surgery of children ankyloglossia,and to explore the effectiveness of the intravenous anesthesia: propofol combined with ketamine.Methods 39 pediatric patients with hypertrophic ankyloglossia were included in this study.After compound anesthetic: propofol with ketamine was given,the plastic surgery was carried out without endotracheal intubation.During the surgery,the respiration,blood pressure,pulse and oxyhemoglobin saturation(SpO2) were monitored.The operation time,intraoperative and postoperative adverse reaction were recorded.Results All cases sustained the anesthesia and the surgery,with good vital signs during surgery.The average operation time was 3 mins,and the average anesthesia time was 20 mins.Low SpO2 appeared in 3 cases during surgery.Postoperative symptoms of nausea,vomiting and laryngospasm with varying degrees,were observed in 12 cases.Conclusions The intravenous anesthesia: propofol combined with ketamine,is a safe and effective method for children ankyloglossia,thus it is worth clinical popularization and application.

Objective To disclose the etiologies of class Ⅲ malocclusion in deciduous dentition due to bottle feeding.Methods Sixty children with a history of bottle feeding in six months after birth were chosen as participants,thirty with normal occlusion(control group) and thirty with class Ⅲ malocclusion(case group).A simulation baby was used for mothers to mimic their feeding gesture when they fed their children during lactation period and the baby′s angulation of head,body,and milk bottle from the horizontal plane were recorded by a customed inclination device.Meanwhile,sucking pressure of each child was measured by a negative pressure transducer.The data were statistically analyzed by SPSS 16.0 software and the significance was set at P<0.05.Results No significant difference in the angles of head,body,and milk bottle between the two groups was found,nor the angle value from bottle to head (P＞0.05).The average sucking pressure value in control group was (20.1±2.7)kPa while the sucking negative pressure in case group was (15.3±3.3)kPa,which was statistically lower than its counterpart (P<0.01).The difference was significant.Conclusions For the children with bottle feeding,the sucking force in the case group is significantly lower than the control group while there is no statistical difference in feeding gesture between these two groups.We conclude that the lower sucking force can be one of the etiologies that cause class Ⅲ malocclusion in deciduous dentition due to bottle feeding and its relationship with feeding gesture needs to be further studied.

Objective To investigate the dimension changes of plaster casts after impression disinfection with various disinfection methods.Methods 54 alginate impressions were made according to a standard model and disinfected with 1〖JP20〗∶〖JP〗100 chlorine containing disinfectant,2% glutaraldehyde disinfection solution and the running water (control group),respectively.Then cast pouring was done to the 54 impressions respectively with ordinary gypsum,anhydrite and super hard plaster.The dimensions of specific positions of the plaster casts were measured and compared with those of the standard model.Twoway ANOVA and Tukey HSD were performed with SPSS 17.0 statistical software.Results After disinfection,the dimensions of the plaster casts were smaller than the standard one; the dimensions of the ordinary gypsum group were close to the standard model.With the same disinfection method,the dimensions of the super hard plaster in glutaral disinfectant group were larger than those of the anhydrite dimensions.In the chlorine group and the control group,the largest was ordinary gypsum,followed by anhydrite and super hard plaster.Statistical analysis showed that cast dimensions were not affected by disinfectant methods,but by various model materials.The largest dimension change was -0.71 mm.Conclusions Chlorine disinfectant and glutaral disinfectant have no significant effect on dimensions of plaster models.

Objective To investigate the clinical features,early diagnosis and treatment of maxillofacial lipoma in order to improve the knowledge of the disease,reduce the misdiagnosis and missed diagnosis. Methods A retrospective analysis of data of 19 cases of maxillofacial lipoma was done with the review of pertinent literature. Results The patients with age above 60 were in the majority,followed by adults under 60,and it rarely occurred to the minors; there was no significant difference in gender; maxillofacial lipoma grew most often in subcutaneous and submucosa,followed by salivary gland,the mucosal surface and muscle,rarely in jaw bones and deep space; there were no specific clinical symptoms,mainly to the accidental discovery; most patients were diagnosed by color Doppler ultrasound,CT examination,and finally confirmed by pathology; tumors were not uniform in size,had complete or incomplete envelope,and clear boundaries with the surrounding normal tissue. It can be easily severed,completely resected and there was no palindromia. Conclusions Maxillofacial lipoma can be diagnosed before surgery by color Doppler ultrasound,CT and MRI examination,and finally confirmed by postoperative pathology. The main method of the treatment is complete operation excision and it won′t recur easily.

Objective To study the clinical application of the keykey attachment used in the bilateral molar missing cases (Kennedy I).Methods 26 patients with bilateral molar missing (Kennedy I) were selected and were repaired with keykey attachment denture.And at the beginning,3 month,6 month,12 month and 24 month,the masticatory efficiency,attachment retentive force,and periodontics index(GI,PD) were investigated and were analysised by stastic software.Results 26 patients treated with keykey way attachment were satisfied with the denture.The masticatory efficiency statistically increased at the time of 3 month,6 month,12 month and 24 month.The retentive force decreased with time,and at the 24 month it had statistically significant compared with the first time.The gingival index (GI) statistically inceased at the time of 3 month.The periodontics probing depths (PD) had no significant change.Conclusions The key-key attachment is a kind of esthetic,stable prosthetics restoration method with high efficiency.

Objective To compare the clinical effects of traditional chisel method and high-speed air turbine drill method of extracting impacted mandibular wisdom teeth,and to evaluate the clinical feasibility and superiority of the high-speed air turbine drill method.Methods 500 patients with impacted mandibular third molars were randomly divided into 2 groups:test group and control group.260 cases (test group) used the highspeed air turbine drill and new instruments to extract the impacted mandibular third molars while other 240 cases (control group) used the bone chiseling and dental crown hacking method to extract the impacted mandibular third molars.The operation time and postoperative complications were observed and recorded to evaluate the effects of the two methods.Results The data showed that the operation time of the test group was obviously shorter than the control group (P＜0.05) .There was significant difference in the degree of pain and limitation of mouth opening degree(P＜0.05) after the operation between two groups.The incidence of alveolalgia of the test group was apparently lower than that of the control group.Conclusions The highspeed air turbine drill and new instruments method of extracting impacted mandibular wisdom teeth can efficiently shorten the operation time,reduce the incidence of complications,so it is worthy to be promoted and widely used.

Objective To observe the changes of the periodontal indexand to compare the periodontal status of adults treated with fixed orthodontic appliances and removable aligners.Methods Eighty 18～32 year-old patients were selected and divided into 2 groups: group A and group B.40 patients in group A wore removable aligners while 40 others in group B wore fixed orthodontic appliances.Periodontal indices(GI,PLI,PD) were examined in both groups before treatment,6 weeks and 6 months after treatment.Results These periodontal indices of fixed orthodontic appliances were significantly greater than those of removable aligners and there was significant difference.Conclusions Compared with fixed orthodontic appliances,removable aligners are more propitious to periodontal health.

Objective To survey on periodontal status of pregnant women and pregnant outcome,and to explore its possible relationship between periodontal infection and preterm birth.Methods 674 pregnant women were selected in Wuxi Maternal and Child HealthCare Hospital during 2012 JanuaryDecember.Women were given periodontal examination.OHIS,SBI,PD and CAL were recorded and pregnant outcome was tracked.The result was analyzed with chisquare test,t test and Spearman correlation analysis.Results ①Periodontitis prevalence of preterm women (36.1%) was higher than that of fullterm pregnant women (21.4%).There was a significant difference in gestational age and birth weight between the two groups (P<0.01); ②OHIS,SBI,PD,CAL of preterm group were higher than fullterm pregnancy group (P<0.05); ③Maternal periodontal index of OHIS,PD,CAL and gestational age was negatively correlated (P<0.05).Conclusions There is a correlation between maternal periodontal infection and preterm birth.Periodontitis among pregnant women is a risk factor for preterm birth.We must pay more attention to oral health care of these pregnant women．

Objective To investigate and analyze the malocclusion and orthodontic conditions of 819 middle school students in Zhoushan city.Methods 819 middle school students were investigated.Individual normal occlusion was set as the criteria.The incidence rate of malocclusion,rectification rate and the primary cause were analyzed statistically.Results The incidence rate of pathoocclusion and rectified rate were 78.8% and 14.5%,respectively.The primary cause of nonrectification was that there was a weak awareness of the harm of malocclusion.Conclusions There is a higher incidence rate of malocclusion with a lower rectification rate among middle students of Zhoushan city.