口腔鳞状细胞癌中NCAPD2的表达水平及生物学功能

doi:10.13591/j.cnki.kqyx.2022.08.003

摘要/Abstract

摘要： 目的探讨非SMC凝集素Ⅰ复合亚基D2(NCAPD2)在口腔鳞状细胞癌(OSCC)中的表达及生物学功能。方法首先应用数据库分析NCAPD2在头颈部鳞状细胞癌(head and neck squamous cell carcinoma, HNSCC)中的表达,然后采用免疫组化法在OSCC标本中进行验证。应用高内涵筛选(high-content screening, HCS)、流式细胞仪及蛋白质印迹法(Western Blot)检测OSCC细胞的增殖、凋亡及凋亡相关蛋白的表达。结果 NCAPD2在HNSCC中高表达,且与HNSCC临床分期、病理分级和淋巴结转移有关。免疫组化显示OSCC组织中的NCAPD2表达显著高于健康口腔组织(P<0.001),且NCAPD2表达与OSCC病理分级之间存在显著相关性。敲减NCAPD2能够抑制OSCC细胞的增殖促进凋亡。此外,BCL-2,p21表达下调,BAX,p53表达上调。结论 NCAPD2有望成为OSCC治疗的一个靶点。

关键词: NCAPD2, 口腔鳞状细胞癌, 增殖, 凋亡

Abstract: Objective To investigate the expression and biological function of non-SMC condensin Ⅰ complex subunit D2 (NCAPD2) in oral squamous cell carcinoma (OSCC). Methods Database was first used to analyze the expression of NCAPD2 in head and neck squamous cell carcinoma (HNSCC), and then immunohistochemistry was used to verify the expression in OSCC specimens. High-content screening (HCS), flow cytometry and Western Blot were used to detect the proliferation and apoptosis of OSCC cells and the expression of apoptosis-related proteins. Results NCAPD2 was highly expressed in HNSCC, and was related to clinical stage, pathological grade and lymph node metastasis of HNSCC. Immunohistochemistry showed that NCAPD2 expression in OSCC tissue was significantly higher than that in healthy oral tissue (P<0.001), and there was a significant correlation between NCAPD2 expression and OSCC pathological grade. Knockdown of NCAPD2 could inhibit the proliferation and promote apoptosis of OSCC cells. In addition, expressions of BCL-2 and p21 were down-regulated, and expressions of BAX and p53 were up-regulated. Conclusion NCAPD2 may be a therapeutic target for OSCC.

Key words: NCAPD2, oral squamous cell carcinoma, proliferation, apoptosis

中图分类号:

R739.8

马萍, 程璐瑶, 金武龙, 王宁, 翟荣萍. 口腔鳞状细胞癌中NCAPD2的表达水平及生物学功能[J]. 口腔医学, 2022, 42(8): 688-693.

MA Ping, CHENG Luyao, JIN Wulong, WANG Ning, ZHAI Rongping. Expression level and biological function of NCAPD2 in oral squamous cell carcinoma[J]. Stomatology, 2022, 42(8): 688-693.

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