Abstract: Objective To investigate the effect of asperosaponinⅥ(ASAⅥ) on the osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hjBMSCs). Methods Specific antigens of hjBMSCs were identified by flow cytometry analysis. The effect of 1×10^-4, 1×10^-5, 1×10^-6, 1×10^-7, 1×10^-8 mol/L ASAⅥ on hjBMSCs' proliferation was detected by CCK8 kit; BCIP/NBT, alizarin red stain (ARS) and western-blot (WB) were used to verify the role of ASAⅥ in promoting hjBMSCs' osteogenic differentiation and to determine the appropriate concentration of ASAⅥ. The effect of ASAⅥ on the osteogenic differentiation of hjBMSCs at mRNA level was further explored by real-time quantitative PCR(RT-PCR). Results Results showed that 1×10^-4mol/L ASAⅥ had obvious toxic effects on hjBMSCs (P<0.01), while the others had no obvious side effect on cell proliferation. Alkaline phosphatase, calcium nodule precipitation, and the expression of osteogenic differentiation-specific proteins were all significantly increased in the 1×10^-5, 1×10^-6 mol/L ASAⅥ group when compared with the control group. The expression of Runx2, OCN and COL-Ⅰ at mRNA level were significantly increased in the 1×10^-5, 1×10^-6 mol/L groups compared to the control group (P<0.01), and the former was slightly predominant. Conclusion The 1×10^-5, 1×10^-6 mol/L ASAⅥ can better promote hjBMSCs' osteogenic differentiation, and 1×10^-5 mol/L group is slightly better.

Key words: asperosaponinⅥ, human jaw bone marrow mesenchymal stem cell, osteogenic differentiation