生长激素释放肽对牙周膜干细胞增殖、凋亡影响的研究

doi:10.13591/j.cnki.kqyx.2022.08.002

Abstract

Abstract: Objective To observe the effects of Ghrelin on proliferation and apoptosis of periodontal ligament stem cells (PDLSCs) and explore its possible mechanism. Methods Surface antigens of cultured PDLSCs were identified by flow cytometry, and the multi-directional differentiation ability of PDLSCs was identified by alizarin red staining and oil red O staining. The effect of Ghrelin on the proliferation of PDLSCs was detected by cell counting kit-8 (CCK-8) assay.The effect of Ghrelin on cell state of PDLSCs was detected by live/dead cell staining. Apoptotic effects of Ghrelin and its receptor GHS-R1α antagonist D-Lys3-GHRP-6 (DLS) on starvation-induced PDLSCs were detected by flow cytometry AnnexinV-FITC/PI double staining and TUNEL staining, respectively. Results Cells cultured in this study were mesenchymal-derived pluripotent stem cells. Moreover, 100 and 200 ng/mL Ghrelin significantly promoted the proliferation of PDLSCs (P<0.05). Moreover, Ghrelin inhibited starvation-induced cell death and apoptosis, and this anti-apoptotic effect was reversed by DLS. Conclusion Ghrelin promotes the proliferation of PDLSCs and inhibits serum-free starvation-induced apoptosis. This process might be related to the binding of Ghrelin to PDLSCs surface receptor GHS-R1α.

Key words: Ghrelin, proliferation, apoptosis, periodontal ligament stem cells

CLC Number:

R781.4

WANG Ting, DU Lingqian. Effects of Ghrelin on proliferation and apoptosis of periodontal ligament stem cells[J]. Stomatology, 2022, 42(8): 681-687.

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Effects of Ghrelin on proliferation and apoptosis of periodontal ligament stem cells

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