低氧预处理人羊膜间充质干细胞来源外泌体在改善血管衰老中的作用研究

doi:10.13591/j.cnki.kqyx.2023.06.003

Abstract

Abstract:

Objective To analyze the role of human amniotic mesenchymal stem cell-derived exosomes(hAMSC-exos) cultured under hypoxic condition in delaying vascular aging. Methods D-galactose(D-gal) stimulation was used to induce subacute senescence in human umbilical vein endothelial cells(HUVEC). Hypoxia-induced hAMSC-exos(Hy hAMSC-exos) was collected to incubate with D-gal-induced HUVEC. Senescence of HUVEC was evaluated by senescence-associated β-galactosidase activity (SA-β-Gal) staining. Protein expression levels of P53, P16 and γH2AX, angiogenic function of HUVEC were detected by scratch assay, transwell migration assay and tube formation assay. Results Subacute senescence model of HUVEC was successfully constructed via D-gal stimulation; SA-β-Gal and detection of protein expression levels of P53, P16 and γH2AX showed that Hy hAMSC-exos could reverse the upregulation of β-Gal, P53, P16 and γH2AX caused by D-gal-induced HUVEC; scratch assay, transwell migration assay and tube formation assay showed that Hy hAMSC-exos could alleviate the weakened angiogenic function of HUVEC induced by D-gal. Conclusion The study indicates that Hy hAMSC-exos could alleviate vascular aging.

Key words: human amniotic mesenchymal stem cell (hAMSC), hypoxia, exosomes, human umbilical vein endothelial cells (HUVEC), senescence

CLC Number:

R781.5

ZHAO Wei,LIU Jinming,YANG Leiting,SHEN Ming,ZHANG Jinglu. Study on the role of human amniotic mesenchymal stem cell-derived exosomes cultured under hypoxic condition in delaying vascular aging[J]. Stomatology, 2023, 43(6): 494-499.

Figures/Tables 5

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References 30

[1]	Folkman J. Angiogenesis[M]//Jaffe EA. Biology of Endothelial Cells. Boston, MA: Springer, 1984:412-428.
[2]	Hankenson KD, Dishowitz M, Gray C, et al. Angiogenesis in bone regeneration[J]. Injury, 2011, 42(6):556-561. doi: 10.1016/j.injury.2011.03.035 pmid: 21489534
[3]	Adell R, Lekholm U, Rockler B, et al. A 15-year study of osseointegrated implants in the treatment of the edentulous jaw[J]. Int J Oral Surg, 1981, 10(6):387-416. doi: 10.1016/s0300-9785(81)80077-4 pmid: 6809663
[4]	Albrektsson T, Wennerberg A. On osseointegration in relation to implant surfaces[J]. Clin Implant Dent Relat Res, 2019, 21(Suppl 1):4-7.
[5]	Brånemark PI, Adell R, Breine U, et al. Intra-osseous anchorage of dental prostheses. Ⅰ. Experimental studies[J]. Scand J Plast Reconstr Surg, 1969, 3(2):81-100. doi: 10.3109/02844316909036699 pmid: 4924041
[6]	Benic GI, Hämmerle CHF. Horizontal bone augmentation by means of guided bone regeneration[J]. Periodontol 2000, 2014, 66(1):13-40. doi: 10.1111/prd.2014.66.issue-1
[7]	Cai YS, Song W, Li JM, et al. The landscape of aging[J]. Sci China Life Sci, 2022, 65(12):2354-2454. doi: 10.1007/s11427-022-2161-3 pmid: 36066811
[8]	Gruber R, Koch H, Doll BA, et al. Fracture healing in the elderly patient[J]. Exp Gerontol, 2006, 41(11):1080-1093. doi: 10.1016/j.exger.2006.09.008 pmid: 17092679
[9]	Haffner-Luntzer M, Hankenson KD, Ignatius A, et al. Review of animal models of comorbidities in fracture-healing research[J]. J Orthop Res, 2019, 37(12):2491-2498. doi: 10.1002/jor.24454 pmid: 31444806
[10]	Pegtel DM, Gould SJ. Exosomes[J]. Annu Rev Biochem, 2019, 88: 487-514. doi: 10.1146/annurev-biochem-013118-111902 pmid: 31220978
[11]	Zhuang L, Xia WZ, Chen DD, et al. Exosomal LncRNA-NEAT1 derived from MIF-treated mesenchymal stem cells protected against doxorubicin-induced cardiac senescence through sponging miR-221-3p[J]. J Nanobiotechnology, 2020, 18(1):157. doi: 10.1186/s12951-020-00716-0
[12]	Yuan N, Ge ZG, Ji WC, et al. Exosomes secreted from hypoxia-preconditioned mesenchymal stem cells prevent steroid-induced osteonecrosis of the femoral head by promoting angiogenesis in rats[J]. Biomed Res Int, 2021, 2021: 6655225.
[13]	Circ-100290 Positively Regulates Angiogenesis Induced by Conditioned Medium of Human Amnion-Derived Mesenchymal Stem Cells Through miR-449a/eNOS and miR-449a/VEGFA Axes[EB/OL].[2022-11-30]. https://www.ijbs.com/v16p2131.htm.
[14]	McCulloch K, Litherland GJ, Rai TS. Cellular senescence in osteoarthritis pathology[J]. Aging Cell, 2017, 16(2):210-218. doi: 10.1111/acel.12562 pmid: 28124466
[15]	Jiang P, Cao P. Topical application of conditioned mediumfrom hypoxically cultured amnion-derived mesenchymal stem cells promotes wound healing in diabetic mice[J]. Plast Reconstr Surg, 2022, 150(4):922e-923e.
[16]	Simunovic F, Finkenzeller G. Vascularization strategies in bone tissue engineering[J]. Cells, 2021, 10(7):1749. doi: 10.3390/cells10071749
[17]	Grosso A, Burger MG, Lunger A, et al. It takes two to tango: Coupling of angiogenesis and osteogenesis for bone regeneration[J]. Front Bioeng Biotechnol, 2017, 5: 68. doi: 10.3389/fbioe.2017.00068
[18]	Atienzar-Aroca S, Flores-Bellver M, Serrano-Heras G, et al. Oxidative stress in retinal pigment epithelium cells increases exosome secretion and promotes angiogenesis in endothelial cells[J]. J Cell Mol Med, 2016, 20(8):1457-1466. doi: 10.1111/jcmm.12834 pmid: 26999719
[19]	Lu GD, Cheng P, Liu T, et al. BMSC-derived exosomal miR-29a promotes angiogenesis and osteogenesis[J]. Front Cell Dev Biol, 2020, 8: 608521. doi: 10.3389/fcell.2020.608521
[20]	Dong X, Lei Y, Yu ZY, et al. Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis[J]. Theranostics, 2021, 11(11):5107-5126. doi: 10.7150/thno.54755 pmid: 33859737
[21]	Hadjiargyrou M, O'Keefe RJ. The convergence of fracture repair and stem cells: Interplay of genes, aging, environmental factors and disease[J]. J Bone Miner Res, 2014, 29(11):2307-2322. doi: 10.1002/jbmr.2373 pmid: 25264148
[22]	Brunauer R, Xia IG, Asrar SN, et al. Aging delays epimorphic regeneration in mice[J]. J Gerontol A Biol Sci Med Sci, 2021, 76(10):1726-1733. doi: 10.1093/gerona/glab131
[23]	Simonsen JL, Rosada C, Serakinci N, et al. Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells[J]. Nat Biotechnol, 2002, 20(6):592-596. doi: 10.1038/nbt0602-592 pmid: 12042863
[24]	Borodkina AV, Deryabin PI, Giukova AA, et al. Social life of senescent cells: What is SASP and why study it?[J]. Acta Naturae, 2018, 10(1):4-14. pmid: 29713514
[25]	Song Y, Zhang C, Zhang JX, et al. Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction[J]. Theranostics, 2019, 9(8):2346-2360. doi: 10.7150/thno.29945 pmid: 31149048
[26]	Yang XC, Yang JX, Lei PF, et al. LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis[J]. Aging, 2019, 11(20):8777-8791. doi: 10.18632/aging.v11i20
[27]	Zuo R, Liu MH, Wang YQ, et al. BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling[J]. Stem Cell Res Ther, 2019, 10(1):30. doi: 10.1186/s13287-018-1121-9 pmid: 30646958
[28]	Kim EC, Kim JR. Senotherapeutics: Emerging strategy for healthy aging and age-related disease[J]. BMB Rep, 2019, 52(1):47-55. doi: 10.5483/BMBRep.2019.52.1.293
[29]	Maas SLN, Breakefield XO, Weaver AM. Extracellular vesicles: Unique intercellular delivery vehicles[J]. Trends Cell Biol, 2017, 27(3):172-188. doi: S0962-8924(16)30179-9 pmid: 27979573
[30]	Lei Q, Gao F, Liu T, et al. Extracellular vesicles deposit PCNA to rejuvenate aged bone marrow-derived mesenchymal stem cells and slow age-related degeneration[J]. Sci Transl Med, 2021, 13(578):eaaz8697. doi: 10.1126/scitranslmed.aaz8697

Study on the role of human amniotic mesenchymal stem cell-derived exosomes cultured under hypoxic condition in delaying vascular aging

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[4]	. Effect of autophagy on proliferation of osteoblasts in hypoxia [J]. , 2018, 38(10): 876-880.
[5]	. Effect of IL-17 on modulating the expression of RANKL and OPG in periodontal ligament cells under hypoxia [J]. , 2017, 37(5): 385-389.
[6]	. Research progress of inflammatory injury in human periodontal ligament cells induced by LPS under hypoxia [J]. , 2017, 37(5): 449-452.
[7]	. Effects of mechanical stress on osteogenic differentiation of human periodontal ligament stem cells under hypoxia [J]. , 2016, 36(5): 478-480.
[8]	. The expression and significance of HIF2α in condyle chondrocytes under different stress loadings [J]. , 2016, 36(2): 101-104.
[9]	. The effect of hypoxia/reoxygenation on the expressions of COX-2 and PGE2 in lipopolysaccharide-induced human periodontal ligament cells [J]. , 2015, 35(9): 714-717.
[10]	. Study on the expression of TLR3 and TLR4 with the stimulation of hypoxia in OSCC cell lines [J]. , 2015, 35(10): 806-809.