钽涂层对hPDLSCs增殖及成骨分化的影响

doi:10.13591/j.cnki.kqyx.2023.05.005

Abstract

Abstract:

Objective To investigate the effect of tantalum-coated surface on the proliferation and ossification of human periodontal ligament stem cells(hPDLSCs). Methods The hPDLSCs were separated and cultured, with the cell identified. After polishing and cleaning of pure titanium specimens, the tantalum coating was prepared by plasma spraying technique after sandblasting and acid etching treatment. The polished titanium specimens(P group)were used as control group, and sandblasted acid-etched titanium(SLA group)and tantalum-coated titanium(Ta group)were used as test groups. The micro-morphology and elemental composition of the titanium surface of each group were analyzed by scanning electron microscopy(SEM)and energy spectrometry(EDS). The hPDLSCs were inhibited on the surface of each group of specimens, and the proliferation activity of cells was measured by CCK-8 assay. After osteogenesis induction culture, alkaline phosphatase activity assay and alizarin red staining were performed. Osteogenic gene expression was detected by qPCR. Results Tantalum coating was successfully prepared on titanium specimens. The hPDLSCs were successfully isolated and identified. The results of CCK-8 experiment showed that after 3, 5 and 7 days of culture, the OD value of Ta group was significantly higher than that of SLA and P groups(P<0.05). The results of ALP assay showed that the difference between the three groups was not statistically significant at day 7;at day 14, the ALP activity in the Ta group was significantly higher than that in the P and SLA groups(P<0.01). Alizarin red staining showed that red calcified nodules were formed on the superficial side of the materials in all three groups, and the mineralized nodules in the SLA and Ta groups were significantly more than those in the P group. The qPCR data indicated that the expression levels of ALP, RUNX2, and OCN genes were significantly higher in the Ta group than in the P group(P<0.01), and the expression of RUNX2 and OCN genes were effectively higher than that in the SLA group(P<0.01). Conclusion Tantalum coating can promote the proliferation and osteogenic differentiation of periodontal stem cells.

Key words: tantalum coating, human periodontal ligament stem cells, osteogenic differentiation

CLC Number:

R783.1

GE Xiao,YU Miao,WU Wei,BI Xiuting,WU Xiaoyan,YU Chen,LI Ti. The effect of tantalum coating on the proliferation and osteogenic differentiation of human periodontal ligament stem cells[J]. Stomatology, 2023, 43(5): 415-420.

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References 30

[1]	Li HL, Yao ZG, Zhang J, et al. The progress on physicochemical properties and biocompatibility of tantalum-based metal bone implants[J]. SN Appl Sci, 2020, 2(4):671. doi: 10.1007/s42452-020-2480-2
[2]	Mani G, Porter D, Grove K, et al. A comprehensive review of biological and materials properties of Tantalum and its alloys[J]. J Biomed Mater Res A, 2022, 110(6):1291-1306. doi: 10.1002/jbm.a.v110.6
[3]	石晓岫, 毛世龙, 刘洋, 等. 钽与钛(合金)骨科材料的差异比较:理化指标及抗菌和成骨能力[J]. 中国组织工程研究, 2021, 25(4):593-599.
[4]	Wang X, Liu WT, Yu XD, et al. Advances in surface modification of tantalum and porous tantalum for rapid osseointegration:A thematic review[J]. Front Bioeng Biotechnol, 2022, 10:983695. doi: 10.3389/fbioe.2022.983695
[5]	吴犇, 陈晓艺, 路怀峰, 等. 等离子喷涂技术在骨植入体表面改性中的研究进展[J]. 中国表面工程, 2021, 34(4):1-11. doi: 10.11933/j.issn.1007-9289.20210410001
[6]	Zhou X, Hu XL, Lin Y. Coating of sandblasted and acid-etched dental implants with tantalum using vacuum plasma spraying[J]. Implant Dent, 2018, 27(2):202-208. doi: 10.1097/ID.0000000000000727
[7]	Zhu Y, Gu YX, Qiao SC, et al. Bacterial and mammalian cells adhesion to tantalum-decorated micro-/ nano-structured titanium[J]. J Biomed Mater Res A, 2017, 105(3):871-878. doi: 10.1002/jbm.a.35953 pmid: 27784134
[8]	张成, 耿藤瑜, 王晶, 等. 纳米形貌钛种植体对骨质疏松大鼠骨结合的影响[J]. 口腔医学研究, 2022, 38(6):545-552. doi: 10.13701/j.cnki.kqyxyj.2022.06.012
[9]	Sayed ME, Mugri MH, Almasri MA, et al. Role of stem cells in augmenting dental implant osseointegration:A systematic review[J]. Coatings, 2021, 11(9):1035. doi: 10.3390/coatings11091035
[10]	DohanEhrenfest DM, Coelho PG, Kang BS, et al. Classification of osseointegrated implant surfaces:Materials, chemistry and topography[J]. Trends Biotechnol, 2010, 28(4):198-206. doi: 10.1016/j.tibtech.2009.12.003 pmid: 20116873
[11]	Ding XL, Xu SL, Li SB, et al. Biological effects of titanium surface charge with a focus on protein adsorption[J]. ACS Omega, 2020, 5(40):25617-25624. doi: 10.1021/acsomega.0c02518 pmid: 33073087
[12]	Dehghanghadikolaei A, Fotovvati B. Coating techniques for functional enhancement of metal implants for bone replacement:A review[J]. Materials (Basel), 2019, 12(11):1795. doi: 10.3390/ma12111795
[13]	Barrak FN, Li SW, Muntane AM, et al. Particle release from implantoplasty of dental implants and impact on cells[J]. Int J Implant Dent, 2020, 6(1):50. doi: 10.1186/s40729-020-00247-1 pmid: 32918144
[14]	Wang X, Ning BY, Pei XB. Tantalum and its derivatives in orthopedic and dental implants:Osteogenesis and antibacterial properties[J]. Colloids Surf B Biointerfaces, 2021, 208:112055. doi: 10.1016/j.colsurfb.2021.112055
[15]	Hao JZ, Li Y, Li BE, et al. Biological and mechanical effects of micro-nanostructured titanium surface on an osteoblasticcell line in vitro and osteointegration in vivo[J]. Appl Biochem Biotechnol, 2017, 183(1):280-292. doi: 10.1007/s12010-017-2444-1
[16]	Winning L, El Karim IA, Lundy FT. A comparative analysis of the osteogenicpotential of dental mesenchymalstem cells[J]. Stem Cells Dev, 2019, 28(15):1050-1058. doi: 10.1089/scd.2019.0023 pmid: 31169063
[17]	Skoog SA, Kumar G, Goering PL, et al. Biological response of human bone marrow-derived mesenchymalstem cells to commercial tantalum coatings with microscale and nanoscalesurface topographies[J]. JOM, 2016, 68(6):1672-1678. doi: 10.1007/s11837-016-1934-x
[18]	蔡洪桢. 钛基人工种植体表面钽涂层改性对其生物学性能影响的实验研究[D]. 北京: 中国人民解放军医学院, 2017.
[19]	Lewallen EA, Trousdale WH, Thaler R, et al. Surface roughness of titanium orthopedic implants alters the biological phenotype of human mesenchymalstromal cells[J]. Tissue Eng Part A, 2021, 27(23/24):1503-1516. doi: 10.1089/ten.tea.2020.0369
[20]	Ma JW, Zan R, Chen WZ, et al. Cell behaviors on surface of pure tantalum with nano-dimpled structure[J]. Rare Met, 2019, 38(6):543-551. doi: 10.1007/s12598-019-01226-1
[21]	Marconi GD, Fonticoli L, Della Rocca Y, et al. Human periodontal ligament stem cells response to titanium implant surface:Extracellular matrix deposition[J]. Biology, 2021, 10(9):931. doi: 10.3390/biology10090931
[22]	Shi JY, Zhang XM, Qiao SC, et al. Enhanced osteointegration of tantalum-modified titanium implants with micro/nano-topography[J]. RSC Adv, 2017, 7(73):46472-46479. doi: 10.1039/C7RA08036K
[23]	An R, Fan PP, Zhou MJ, et al. Nanolamellartantalum interfaces in the osteoblast adhesion[J]. Langmuir, 2019, 35(7):2480-2489. doi: 10.1021/acs.langmuir.8b02796
[24]	姜铭坤, 何亚茹, 吴文慧. 钛钽生物梯度材料在成骨过程中对碱性磷酸酶的影响[J]. 当代医学, 2021, 27(6):39-41.
[25]	Piglionico S, Bousquet J, Fatima N, et al. Porous tantalum VS. titaniumimplants:Enhanced mineralized matrix formation after stem cells proliferation and differentiation[J]. J Clin Med, 2020, 9(11):3657. doi: 10.3390/jcm9113657
[26]	Liu XY, Song XB, Zhang P, et al. Effects of nano tantalum implants on inducing osteoblast proliferation and differentiation[J]. Exp Ther Med, 2016, 12(6):3541-3544. doi: 10.3892/etm.2016.3801 pmid: 28101149
[27]	Vimalraj S. Alkaline phosphatase:Structure, expression and its function in bone mineralization[J]. Gene, 2020, 754:144855. doi: 10.1016/j.gene.2020.144855
[28]	Komori T. Functions of osteocalcin in bone, pancreas, testis, and muscle[J]. Int J Mol Sci, 2020, 21(20):7513. doi: 10.3390/ijms21207513
[29]	Komori T. Whole aspect of Runx2 functions in skeletal develop-ment[J]. Int J Mol Sci, 2022, 23(10):5776. doi: 10.3390/ijms23105776
[30]	Qian H, Lei T, Ye ZM, et al. From the performance to the essence:The biological mechanisms of how tantalum contributes to osteogenesis[J]. Biomed Res Int, 2020, 2020:5162524.

基因名称	上游引物(5'—3')	下游引物(5'—3')
ALP	GGCGGTGAACGAGAGAATGT	GGACGTAGTTCTGCTCGTGG
OCN	TCACACTCCTCGCCCTATTG	CTCTTCACTACCTCGCTGCC
RUNX2	GGAGTGGACGAGGCAAGAGT	AGGCGGTCAGAGAACAAACT
GADPH	GCACCGTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA

分组	1 d	3 d	5 d	7 d
P组	0.15±0.01	0.25±0.07	0.35±0.06	1.02±0.04
SLA组	0.15±0.00	0.27±0.06	0.40±0.03^#	1.28±0.08^#
Ta组	0.16±0.00	0.39±0.01^*#	0.64±0.05^*#	1.70±0.21^*#

分组	7 d	14 d
P组	7.80±2.71	51.58±0.72
SLA组	9.90±5.43	53.87±7.59
Ta组	15.08±6.19	73.58±5.20^**##

组别	ALP	RUNX2	OCN
P组	1.00±0.24	1.00±0.09	1.00±0.15
SLA组	1.47±0.17^*	1.30±0.10^*	1.49±0.10^**
Ta组	1.86±0.11^**	2.44±0.30^**##	1.88±0.16^**##

The effect of tantalum coating on the proliferation and osteogenic differentiation of human periodontal ligament stem cells

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