初级纤毛在口腔黏膜创伤愈合中的作用研究

doi:10.13591/j.cnki.kqyx.2023.07.002

Abstract

Abstract:

Objective To investigate the correlation between ageing changes in primary cilia and oral mucosal wound healing. Methods A wound model was constructed for buccal oral mucosa of mice at different ages, and samples were collected at 1, 3 and 5 days after modeling. The healing process was observed by HE staining, the tissue remodeling by MASSON staining, the re-epithelialization process by immunofluorescence staining, and the difference of cell proliferation level during the wound healing process by EdU labeling. By constructing mice with specific knockout of the primary cilia key molecule Ift140 in oral mucosal epithelial, the difference of oral mucosal wound healing speed between knockout and control mice was observed. Results The oral mucosal epithelium of aged mice had decreased self-renewal ability and the occurrence rate of primary cilia was reduced. Mucosal healing was delayed after wound, and the proliferation rate of keratinocytes was reduced and the migration rate to the wound bed was slowed. Conditional knockdown of Ift140 in oral mucosal epithelial K14-positive cells interfered with primary cilia formation and delayed the wound healing process. Conclusion Age-related changes in primary cilia of mouse oral mucosal epithelium delay oral mucosal wound healing.

Key words: oral mucosa, wound healing, re-epithelialization, primary cilia, aging

CLC Number:

R781.59

WANG Xinyu, SUN Yao. Study on the role of primary cilia in the healing of oral mucosal wound[J]. Stomatology, 2023, 43(7): 584-591.

Figures/Tables 5

References 32

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Study on the role of primary cilia in the healing of oral mucosal wound

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