褪黑素对人牙周膜细胞成骨分化能力的影响

Abstract

Abstract: Objective　 To evaluate the effects of melatonin on the function of osteogenic differentiation of human periodontal ligament cells (hPDLCs).　Methods　 Melatonin was added into hPDLCs as experimental group. The blank control group was not added with melatonin. The effect of different concentrations of melatonin on proliferation of hPDLCs was assessed through MTT assay. Alkaline phosphatase (ALP) activity assay, ALP staining, Alizarin red S staining, Real-time PCR and Western Blot were used to observe the ALP activity, formation of mineral nodules, mRNA and protein expression of hPDLCs, respectively.　Results　 Compared to the control group, the proliferation of hPDLCs in the melatonin groups decreased. Melatonin was shown to enhance the ALP activity and expression of hPDLCs. The melatonin groups were observed more mineral nodules compared to the control group. Real-time PCR and Western Blot results showed that the mRNA and protein expression of Col-I, Runx2 and OSX increased by melatonin in different concentration.　Conclusions　 Melatonin has a promoting effect on the osteogenic differentiation of hPDLCs, and it has further research on the value of periodontal regenerative therapy.

Key words: Melatonin, Periodontitis, Osteogenesis, Human periodontal ligament cells

CLC Number:

R349.5

References 25

[1]	Ira B. Lamster,Michael Pagan. Periodontal disease and the metabolic syndrome[J]. International Dental Journal,2017,67(2).
[2]	Pihlstrom B L , Michalowicz B S , Johnson N W . Periodontal diseases.[J]. Lancet, 2005, 366(9499):1809-1820.
[3]	Lee S I , Lee D W , Yun H M , et al. Expression of thymosin beta-4 in human periodontal ligament cells and mouse periodontal tissue and its role in osteoblastic/cementoblastic differentiation[J]. Differentiation, 2015, 90(1-3):16-26.
[4]	Su Fang,Liu Shi-Sen,Ma Jun-Li et al. Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells.[J] .Stem Cell Res Ther, 2015, 6: 22.
[5]	Ishikawa Isao,Iwata Takanori,Washio Kaoru et al. Cell sheet engineering and other novel cell-based approaches to periodontal regeneration.[J] .Periodontol. 2000, 2009, 51: 220-238.
[6]	Isaka J,Ohazama A,Kobayashi M et al. Participation of periodontal ligament cells with regeneration of alveolar bone.[J] .J. Periodontol., 2001, 72: 314-323.
[7]	Maria P , López-Pe?a Mónica, González-Cantalapiedra Antonio, et al. Melatonin: A Review of Its Potential Functions and Effects on Dental Diseases[J]. International Journal of Molecular Sciences, 2017, 18(4):865-.
[8]	Wang B , Wen H , Smith W , et al. Regulation effects of melatonin on bone marrow mesenchymal stem cell differentiation[J]. Journal of Cellular Physiology, 2019, 234(2):1008-1015.
[9]	Suzuki N , Hattori A . Melatonin suppresses osteoclastic and osteoblastic activities in the scales of goldfish[J]. Journal of Pineal Research, 2002, 33(4):253-258.
[10]	Arabac? Taner,Kermen Eda,?zkanlar Se?ki, et al. Therapeutic Effects of Melatonin on Alveolar Bone Resorption After Experimental Periodontitis in Rats: A Biochemical and Immunohistochemical Study[J]. Journal of Periodontology, 2015, 86(7):874-881.
[11]	Cutando A , Antonio López-Valverde, Rafael Gómez de Diego, et al. Effect of topical application of melatonin to the gingiva on salivary osteoprotegerin, RANKL and melatonin levels in patients with diabetes and periodontal disease[J]. Odontology, 2014, 102(2):290-296.
[12]	Pihlstrom B L, Michalowicz B S, Johnson N W. Periodontal diseases.[J]. Lancet, 2005, 366(9499):1809-1820.
[13]	Ogawa K, Miyaji H, Kato A, et al. Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs [J]. J Periodontal Res, 2016, 51(6): 758-767
[14]	HAN J, MENICANIN D, GRONTHOS S, et al. Stem cells, tissue engineering and periodontal regeneration[J]. Aust Dent J, 2014, 59(Suppl 1): 117-130.
[15]	Xia Y , Tang H N , Wu R X , et al. Cell Responses to Conditioned Media Produced by Patient-Matched Stem Cells Derived From Healthy and ‘Inflamed’ Periodontal Ligament Tissues[J]. Journal of Periodontology, 2015:1-21.
[16]	Cutando A , Jose Aneiros-Fernández, Antonio López-Valverde, et al. A new perspective in Oral health: Potential importance and actions of melatonin receptors MT1, MT2, MT3, and RZR/ROR in the oral cavity[J]. Archives of Oral Biology, 2011, 56(10):944-950.
[17]	Balci Yuce H , Karatas O , Aydemir Turkal H , et al. The Effect of Melatonin on Bone Loss, Diabetic Control and Apoptosis in Diabetic Rats With Ligature-Induced Periodontitis[J]. Journal of Periodontology, 2015:1-16.
[18]	Roth J A, Kim B G, Lin W L, et al. Melatonin promotes osteoblast differentiation and bone formation[J]. Journal of Biological Chemistry, 1999, 274(31):22041-22047.
[19]	Hyung K , Ha K , Moon-Kyoung B , et al. Suppression of Osteoclastogenesis by Melatonin: A Melatonin Receptor-Independent Action[J]. International Journal of Molecular Sciences, 2017, 18(6):1142-.
[20]	Niu L N , Sun J Q , Li Q H , et al. Intrafibrillar-silicified collagen scaffolds enhance the osteogenic capacity of human dental pulp stem cells[J]. Journal of Dentistry, 2014, 42(7):839-849.
[21]	Komori, Toshihisa. Signaling networks in RUNX2‐dependent bone development[J]. Journal of Cellular Biochemistry, 2015, 112(3):750-755.
[22]	Artigas N , Urena C , Rodriguez-Carballo E , et al. Mitogen-activated Protein Kinase (MAPK)-regulated Interactions between Osterix and Runx2 Are Critical for the Transcriptional Osteogenic Program[J]. Journal of Biological Chemistry, 2014, 289(39):27105-27117.
[23]	马勇, 丁体龙, 袁福华, et al. 褪黑素对自身免疫性肝炎大鼠肝星状细胞透明质酸、Ⅲ型前胶原分泌及Ⅰ型胶原mRNA表达的抑制作用[J]. 肝脏, 2008(3).
[24]	李盛楠, 霍波, 张丁. 定量观察力值对人牙周膜细胞骨改建相关细胞因子表达的影响[J]. 中华口腔正畸学杂志, 2015, 22(2):100-103.
[25]	王立媛, 刘大勇, 贾智, et al. 人脱落乳牙干细胞骨向分化相关分子Runx2的动态表达[J]. 中国组织工程研究, 2014, 18(15):2345-2350.

The effects of melatonin on promoting the osteogenesis of human periodontal ligament cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TIAN Shilin, CHEN Xiaotao. Research progress of oxidative stress level and iron metabolism in periodontitis [J]. Stomatology, 2023, 43(6): 552-555.
[2]	SU Endian, CHEN Sulin, GUO Jianbin, HUANG Wenxiu, WU Dong. Effect of a space stabilizing device on the capacity for osteogenesis in the bone-grafted area [J]. Stomatology, 2023, 43(3): 233-236.
[3]	XIE Yongmei, GE Huabing, FENG Jie, LIU Qi, ZHANG Ning, ZHONG Xiaoli. A short-term efficacy evaluation of biological antibacterial peptides in the adjuvant therapy of stageⅢ periodontitis [J]. Stomatology, 2023, 43(3): 237-241.
[4]	WANG Dexu, LUO Xiaojie, CHEN Xiaotao. Research progress of research on the effect of quorum-sensing system on periodontal pathogens [J]. Stomatology, 2023, 43(3): 267-272.
[5]	ZHOU Huirong, LIN Xiaoping. Research advances in correlation between chronic periodontitis and prostatic diseases [J]. Stomatology, 2023, 43(3): 273-277.
[6]	LEI Gang, WEI Xin, YAN Ming, ZHOU Lili, YU Jinhua, WANG Juan, WU Jintao. Clinical effects of iRoot BP plus and mineral trioxide aggregate in treatment of apical periodontitis with open apical permanent teeth [J]. Stomatology, 2023, 43(2): 118-124.
[7]	ZHANG Chaoying, GONG Jiaxing, YU Mengfei, QIAN Ying, ZHU Ziyu, LU Kejie, WANG Huiming. Application progress of alveolar ridge preservation in patients with tooth extracted due to periodontitis [J]. Stomatology, 2023, 43(2): 159-165.
[8]	SUN Yuezhang,WANG Qi. Morphological and immune characteristics of periodontal tissues under hyperglycemia [J]. Stomatology, 2023, 43(2): 153-158.
[9]	DAI Anna,DING Peihui. Periodontal therapy for stage Ⅳ grade C generalized periodontitis: A case report of 7-year follow-up [J]. Stomatology, 2023, 43(2): 145-152.
[10]	ZHANG Qian, WANG Chang, LIANG Chen, QU Xingyuan, LIU Yue, YAN Baojun, WANG Lei. Progress of research on application of chondroitin sulfate in osteogenic repair materials [J]. Stomatology, 2023, 43(1): 88-91.
[11]	LIU Yuqing, GONG Yao, ZHAO Sufeng, DAI Li. Regulation effect of miR-155 on osteogenic differentiation of periodontal ligament stem cells in inflammatory microenvironment [J]. Stomatology, 2022, 42(9): 790-795.
[12]	GENG Ying, MA Lu, FENG Zehua, WANG Xiaoqian, XU Yan, LI Lu. Evaluation of periodontal minimally invasive non-surgical therapy for the treatment of upper anterior teeth of patients with thin gingival phenotype [J]. Stomatology, 2022, 42(9): 807-812.
[13]	ZHANG Shuting, WU Yaxing, LIU Cuicui, LI Chenchen, ZHANG Jing. Research progress of exosomes in osteogenic differentiation of bone marrow mesenchymal stem cells and in periodontal regeneration [J]. Stomatology, 2022, 42(8): 741-744.
[14]	DING Yu, YU Lüfeng, ZOU Derong. The cause and treatment of congenital condyle deformity [J]. Stomatology, 2022, 42(8): 745-749.
[15]	LU Lijie, MIAO Lei, DENG Yu, PAN Yaping. Restoration of oral function by dental implantation combined with free gingival graft in a patient with distal-extension absence and periodontitis:A case report [J]. Stomatology, 2022, 42(8): 727-731.