ATF6在巨噬细胞介导的牙周炎进程中的作用研究

Abstract

Abstract:

Objective To investigate the role of activating transcription factor 6(ATF6) in macrophage-mediated periodontal inflammation and alveolar bone resorption. Methods An inflammation model was established by stimulating RAW264.7 cells with lipopolysaccharide (LPS). RT-qPCR was used to detect changes in the expression of inflammatory factors and ATF6. The function of ATF6 was further validated using siRNA knockdown. Macrophage-specific ATF6 knockout mice(ATF6^fl/fl; Cx3cr1^CreERT2) were generated. Experimental periodontitis was induced using the silk ligature method. Alveolar bone resorption and inflammation levels were evaluated by Micro-CT, HE staining, and bone morphometric analysis. Results As shown in experiments, LPS stimulation significantly upregulated ATF6 expression in macrophages and markedly increased the level of inflammatory. Knockdown of ATF6 significantly inhibited the expression of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in LPS-induced macrophages. Consistently, studies demonstrated that macrophage-specific deletion of ATF6 notably attenuated alveolar bone loss induced by periodontitis and improved the bone volume fraction(BV/TV). Conclusion Macrophage-derived ATF6 plays a critical pro-inflammatory role in the progression of periodontitis. Reduction of its expression attenuates inflammatory responses and alveolar bone resorption.

Key words: periodontitis, ATF6, macrophages, bone resorption, inflammatory response

CLC Number:

R781.4

CHEN Jian, DAI Shuyue, ZHANG Qinyu, ZHOU Yiyi, BI Mengfei, SHEN Ming. The role of ATF6 in macrophage-mediated periodontitis progression[J]. Stomatology, 2026, 46(6): 416-421.

Figures/Tables 6

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References 20

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引物名称	引物序列(5'-3')
β-actin上游引物	GTGACGTTGACATCCGTAAAGA
β-actin下游引物	GCCGGACTCATCGTACTCC
ATF6上游引物	CACCCATCCGAGTTGTGAGG
ATF6下游引物	GCAAACAACGTCGACTCCCA
iNOS上游引物	CAACAGGGAGAAAGCGCAAA
iNOS下游引物	GGATTCTGGAACATTCTGTGCTG
IL-1β上游引物	TGCCACCTTTTGACAGTGATG
IL-1β下游引物	ATGTGCTGCTGCGAGATTTG
IL-6上游引物	TGATGGATGCTACCAAACTGGA
IL-6下游引物	TGTGACTCCAGCTTATCTCTTGG
TNF-α上游引物	GGTGCCATGTCTCAGCCTCTT
TNF-α下游引物	GCCATAGAACTGATGAGAGGGAG
ATF6^fl/fl上游引物	ACCAGTGGTTAGGACTTAGG
ATF6^fl/fl下游引物	AGAAGTTACACGGCACACAG
Cx3cr1^CreERT2通用引物	AAGACTCACGTGGACCTGCT
Cx3cr1^CreERT2突变上游引物	CGGTTATTCAACTTGCACCA
Cx3cr1^CreERT2野生型上游引物	AGGATGTTGACTTCCGAGTTG

The role of ATF6 in macrophage-mediated periodontitis progression

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