长链非编码RNA LINC00958在头颈部鳞状细胞癌的表达和初步研究

doi:10.13591/j.cnki.kqyx.2023.11.002

Abstract

Abstract:

Objective To explore the expression and role of LncRNA LINC00958 in head and neck squamous cell carcinoma(HNSCC). Methods The expression of LINC00958 in HNSCC and its effect on survival and prognosis were analyzed using TCGA database. HNSCC cell lines CAL27 and HN6 were cultured in vitro, and the transfected control group and knockdown group(si-LINC00958)plasmid were detected by real-time quantitative PCR and western blotting(WB)assay. The cell proliferation ability was detected by CCK-8 assay. The changes of cell migration and invasion ability were detected by transwell assay. The expressions of transfer-related proteins E-cadherin, N-cadherin and vimentin were detected by WB assay. The binding sites of LINC00958 and miR-877-3p were predicted by TargetScan database, and verified by double luciferase reporter gene assay. The effects of miR-877-3p on proliferation, migration and invasion of HNSCC cells were analyzed. Results LINC00958 was highly expressed in HNSCC and affected the overall survival rate(P<0.05). Compared with the control group(si-NC), the proliferation, migration and invasion of cells in si-LINC00958 group were decreased; the expression of E-cadherin was decreased, and the expression of N-cadherin and Vimentin was increased(P<0.05). The TargetScan database showed that miR-877-3p was the target gene of LINC00958, and the targeting relationship between miR-877-3p and LINC00958 was verified by double luciferase reporter gene assay. Compared with the control group(NC), cell proliferation, migration and invasion in miR-877-3p mimics group were significantly decreased(P<0.05). Conclusion LINC00958 promotes the proliferation, migration and invasion of HNSCC by targeting miR-877-3p.

Key words: LINC00958, miR-877-3p, head and neck squamous cell carcinoma, proliferation, migration

CLC Number:

R739.85

JI Huan, LI Meng, YAO Enhui, ZHONG Yi, ZHANG Yuyao, WU Heming, LI Bin. Study on the expression of LncRNA LINC00958 in head and neck squamous cell carcinoma[J]. Stomatology, 2023, 43(11): 968-974.

Figures/Tables 11

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References 20

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引物名	序列(3'—5')
LINC00958	上游:CCATTGAAGATACCACGCTGC 下游:GGTTGTTGCCCAGGGTAGTG
E-cadherin	上游:GCCTTATGATTCTCTGCTCGTG 下游:GCCCCATTCGTTCAAGTAGTC
N-cadherin	上游:GTGAGCCTGCAGATTTTAAGGTG 下游:GTTGGCTTCAGGCTCATTTTACT
Vimentin	上游:CTGGATTCACTCCCTCTGGTT 下游:TCGTGATGCTGAGAAGTTTCGTT
GAPDH	上游:GTCAACGGATTTGGTCTGTATT 下游:AGTCTTCTGGGTGGCAGTGAAT
U6	上游:AGAGAGATTACATGGCCCCT 下游:CTAATGTCACGCACGATTCT
miR-877-3p	上游:GGGTGTCCTCTTCTCCCTC 下游:CAGTGCGTGTCGTGGAGT

Study on the expression of LncRNA LINC00958 in head and neck squamous cell carcinoma

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