未控制的2型糖尿病伴Ⅲ/Ⅳ期牙周炎患者龈下菌斑菌群结构及代谢功能分析

doi:10.13591/j.cnki.kqyx.2025.12.002

Abstract

Abstract:

Objective To explore the differences and metabolic function in subgingival microbiome in Stage Ⅲ/Ⅳ periodontitis with and without uncontrolled type 2 diabetes mellitus. Methods A total of 30 subjects with Stage Ⅲ/Ⅳ periodontitis were included in this study, including 15 cases of diabetes (DM) group and 15 cases of normal blood glucose (NDM) group. Basic information of the subjects was recorded and subgingival plaque was collected at periodontal pockets of probing depth (PD)≥ 5 mm before periodontal treatment. DNA was extracted to amplify the highly variable regions V3-V4 of 16S rRNA, and sequencing, bioinformatics and statistical analysis was performed on the constructed library. Results This study obtained a total of 2 252 397 high-quality sequences and 4 935 feature sequences. The species classification annotation included 18 phyla, 39 classes, 72 orders, 120 families, 274 genera, and 508 species. Alpha diversity analysis showed a significantly higher Shannon index in DM group. The β diversity analysis showed that there was a certain similarity in the microbial species composition between the two groups. At the phylum level, there was one significant difference in the microbial community between two groups, Chloroflexi, a non-dominant bacterium, while there was no significant difference between dominant bacteria. At the genus level, the relative abundance of Filifactor, Rikenellaceae_RC9_gut_group, and Tannerella was higher in the NDM group, while the relative abundance of Leptotrichia and Selenomonas was higher in the DM group. Functional prediction of subgingival microbiota revealed 26 significantly up-regulated metabolic pathways in the DM group. Conclusion In patients with uncontrolled type 2 diabetes combined with Stage Ⅲ/Ⅳ periodontitis, the subgingival plaque has more abundant microbial species, and the relative abundance of Leptotrichia and Selenomonas at the genus level is significantly higher, while the metabolic level is up-regulated.

Key words: type 2 diabetes mellitus, Stage Ⅲ/Ⅳ periodontitis, subgingival plaque, 16S rRNA sequencing

CLC Number:

R780.2

FAN Yadan, SONG Zhongchen, LIAO Yue, CHENG Lan, GE Linhua. Alterations and metabolic function in subgingival microbiome in Stage Ⅲ/Ⅳ periodontitis patients with uncontrolled type 2 diabetes mellitus[J]. Stomatology, 2025, 45(12): 887-894.

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References 33

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Alterations and metabolic function in subgingival microbiome in Stage Ⅲ/Ⅳ periodontitis patients with uncontrolled type 2 diabetes mellitus

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