芍药苷对人颌骨骨髓间充质干细胞体外增殖及成骨分化的影响

doi:10.13591/j.cnki.kqyx.2026.02.006

摘要/Abstract

摘要：

目的探究芍药苷(paeoniflorin,PF)对人颌骨骨髓间充质干细胞(human jaw bone marrow mesenchymal stem cells,hJBMMSCs)增殖及成骨分化的影响及相关机制。方法采用组织块法分离培养hJBMMSCs,根据实验要求进行相关条件培养,采用CCK-8法检测不同浓度(1、5、10、50、100 μmol/L)PF对hJBMMSCs增殖能力的影响;通过碱性磷酸酶(ALP)检测分析、茜素红S染色、RT-qPCR和Western blot探讨PF对hJBMMSCs成骨分化及PI3K/AKT信号通路相关蛋白表达水平的影响。结果 10 μmol/L PF显著促进hJBMMSCs增殖,而100 μmol/L PF有明显细胞毒性;其中,10 μmol/L浓度条件下hJBMMSCs各成骨相关mRNA和蛋白表达水平均明显上升,并上调p-PI3K表达水平,而PI3K抑制剂干预后,hJBMMSCs的成骨能力显著下降。结论 10 μmol/L PF可能通过调节PI3K/AKT信号通路以促进hJBMMSCs的成骨分化。

关键词: 芍药苷, 人颌骨骨髓间充质干细胞, 成骨分化, PI3K/AKT信号通路

Abstract:

Objective To investigate the effect of paeoniflorin (PF) on the proliferation and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) and its related mechanisms. Methods hJBMMSCs were isolated and cultured by tissue block method, cultured according to experimental requirements, and the effects of different concentrations (1, 5, 10, 50, 100 μmol/L) PF on the proliferation of hJBMMSCs were detected by CCK-8 method. The effects of PF on the osteogenic differentiation of hJBMMSCs and the expression of proteins related to the PI3K/AKT signaling pathway were investigated by alkaline phosphatase (ALP), alizarin red S staining, RT-qPCR and Western blot. Results PF (10 μmol/L) significantly promoted the proliferation of hJBMMSCs, while 100 μmol/L PF had obvious cytotoxicity. Among them, the expression levels of various osteogenic-related mRNA and protein in hJBMMSCs increased significantly under the condition of 10 μmol/L concentration, and the expression level of p-PI3K was up-regulated. After intervention with PI3K inhibitors, the osteogenic capacity of hJBMMSCs decreased significantly. Conclusion PF (10 μmol/L) may promote the osteogenic differentiation of hJBMMSCs by regulating the PI3K/AKT signaling pathway.

Key words: paeoniflorin, human jaw bone marrow mesenchymal stem cells, osteogenic differentiation, PI3K/AKT signaling pathway

中图分类号:

R780.2

赖敏琴, 胡宗浩, 秦子顺, 殷丽华. 芍药苷对人颌骨骨髓间充质干细胞体外增殖及成骨分化的影响[J]. 口腔医学, 2026, 46(2): 118-125.

LAI Minqin, HU Zonghao, QIN Zishun, YIN Lihua. Effects of paeoniflorin on proliferation and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells in vitro[J]. Stomatology, 2026, 46(2): 118-125.

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基因	正向引物(5'-3')	反向引物(5'-3')
runx2	CTACTATGGCACTTCGTCAGGAT	ATCAGCGTCAACACCATCATT
alp	CTCCTCGGAAGACACTCTGACC	CTGCGCCTGGTAGTTGTTGTG
ocn	AGCCACCGAGACACCATGAG	GCTCCCAGCCATTGATACAGG
gapdh	GGAAGCTTGTCATCAATGGAAATC	TGATGACCCTTTTGGCTCCC