负载锶离子水凝胶促进人牙周膜干细胞成骨分化的转录组学特征及分子网络初探

doi:10.13591/j.cnki.kqyx.2026.05.006

摘要/Abstract

摘要：

目的探讨负载锶离子可注射光固化水凝胶（PLSr²⁺@HAMA）对人牙周膜干细胞（human periodontal ligament stem cells，hPDLSCs）成骨分化相关表型的影响，并基于转录组学分析其潜在分子调控网络特征。方法采用CCK-8法评价水凝胶对hPDLSCs细胞增殖的影响；通过茜素红S染色（alizarin red S，ARS）、实时定量PCR（RT-qPCR）及Western blot（WB）检测成骨相关指标的变化。进一步进行RNA-seq测序，利用生物信息学分析工具对差异表达基因开展功能富集与蛋白-蛋白相互作用网络分析。结果 PLSr²⁺@HAMA 水凝胶具有良好的生物相容性，ARS染色显示其诱导矿化结节沉积趋势更明显；RT-qPCR与WB提示实验组碱性磷酸酶（alkaline phosphatase，ALP）与骨形态发生蛋白2（bone morphogenetic protein 2，BMP2）表达水平呈上调趋势。转录组分析显示，差异表达基因主要与细胞外基质调控、信号转导及细胞应答等过程相关，并筛选得到多种与成骨及基质调控相关的候选枢纽基因。结论 PLSr²⁺@HAMA 水凝胶可促进hPDLSCs成骨分化，并诱导与成骨相关的转录应答变化，为其在牙周组织再生研究中的进一步应用提供实验依据。

关键词: 水凝胶, 成骨分化, 转录组学, 人牙周膜干细胞, 锶离子

Abstract:

Objective To investigate the effect of an injectable photo-crosslinking hydrogel loaded with strontium ions （PLSr²⁺@HAMA） on osteogenic differentiation-related phenotypes of human periodontal ligament stem cells （hPDLSCs） and to analyze its potential molecular regulatory features through transcriptomic analysis. Methods Cell viability of hPDLSCs was evaluated using the CCK-8 assay. Alizarin Red S （ARS） staining，real-time quantitative PCR （RT-qPCR），and Western blot （WB） were used to detect changes in osteogenic-related markers. RNA-seq was performed，and bioinformatic analysis tools were used to conduct functional enrichment and protein-protein interaction network analysis of differentially expressed genes. Results PLSr²⁺@HAMA hydrogel did not significantly affect cell proliferation. ARS staining showed enhanced mineralization nodule formation. RT-qPCR and WB results indicated increased expression of alkaline phosphatase （ALP） and bone morphogenetic protein 2 （BMP2）. Transcriptomic analysis revealed that differentially expressed genes were mainly associated with extracellular matrix regulation，signal transduction，and cellular responses. Several hub genes related to osteogenesis and matrix regulation were identified. Conclusion PLSr²⁺@HAMA hydrogel promotes osteogenic differentiation of hPDLSCs and induces changes in osteogenic-related transcriptional responses，providing experimental support for its application in periodontal tissue regeneration.

Key words: hydrogels, osteogenesis, transcriptomics, human periodontal ligament stem cells, strontium ions

中图分类号:

R781.4

戴佳韵, 王星阳, 周朵, 卢逸瑶, 王娟. 负载锶离子水凝胶促进人牙周膜干细胞成骨分化的转录组学特征及分子网络初探[J]. 口腔医学, 2026, 46(5): 356-362.

DAI Jiayun, WANG Xingyang, ZHOU Duo, LU Yiyao, WANG Juan. Strontium ion-loaded hydrogel promotes osteogenic differentiation of hPDLSCs： Transcriptomics and molecular network analysis[J]. Stomatology, 2026, 46(5): 356-362.

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基因名称	引物序列（5'→3'）
BMP2	F：AAGCCAAACACAAACAGCGG R：ATCAGCCAGAGGAAAAGGGC
ALP	F：CTTGCTGGTGGAAGGAGGCAGG R：CACGTCTTCTCCACCGTGGGTC
GAPDH	F：GATGGTGAAGGTCGGTGTG R：GAGGTCAATGAAGGGGTCG