浓缩生长因子促进大鼠下颌骨联合部成骨作用的初步研究

doi:10.13591/j.cnki.kqyx.2023.11.003

Abstract

Abstract:

Objective To investigate the effect of CGF on promoting the osteogenesis of the mandible symphysis in rats, and to preliminarily explore the possible mechanism. Methods A total of 24 rats were randomly divided into three groups: Collagen group, Collagen + CGF group, CGF group. Three and six months after operation, the rats were killed in two batches. The in vitro specimens were photographed by micro-CT, and the radiological manifestations of each group were compared by three-dimensional reconstruction and cross-section. Statistical analysis was conducted according to standard scores. The specimens were decalcified after micro-CT, and histological sections were made to compare differences among the groups. Results Statistical analysis showed that at the 3rd month, the score of CGF+Collagen group was significantly higher than that of CGF group and Collagen group(P=0.026 6,P=0.026 6), but there was no significant difference between CGF group and Collagen group. At the 6th month, CGF+Collagen group had a significant advantage compared with CGF group and Collagen group(P=0.002 1,P=0.010 1). Collagen group seemed to be better than CGF group, but there was no statistical difference. Through HE staining, it was found that the score of CGF+Collagen group was significantly better than that of CGF group and Collagen group at the 3rd month(P=0.003 8,P=0.003 8), but there was no significant difference between CGF group and Collagen group. At the 6th month, compared with CGF group and Collagen group, the score of CGF+Collagen group was still significantly higher than that of Collagen group (P=0.008 2,P=0.019 4). The score of Collagen group was slightly higher than that of CGF group, but there was no significant difference. Conclusion The combination of CGF and Collagen can significantly promote the healing of bone defects, but the effect of CGF alone on bone defects is not ideal.

Key words: rat mandibular symphysis, concentrated growth factor, osteogenesis

CLC Number:

R782.23

LU Peng, CHEN Zhihong, YANG Yuemei, YIN Zhonghao, WU Fuli, SONG Xiaomeng, WU Yunong. A preliminary study on the effect of CGF on osteogenesis in the rat mandibular symphysis[J]. Stomatology, 2023, 43(11): 975-980.

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References 23

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得分值	CGF	CGF+胶原骨	胶原骨
第3个月时
0分	2	0	2
1分	2	1	2
2分	0	3	0
3分	0	0	0
第6个月时
0分	2	0	1
1分	2	0	2
2分	0	1	1
3分	0	3	0

检测时间	CGF	CGF+胶原骨	胶原骨
3个月	0.27±0.05	0.41±0.05	0.35±0.02
6个月	0.29±0.06	0.48±0.03	0.38±0.05

检测时间	CGF	CGF+胶原骨	胶原骨
3个月	(38.28±8.27)%	(63.34±5.79)%	(46.19±5.97)%
6个月	(40.28±9.30)%	(78.28±6.85)%	(51.37±6.40)%

A preliminary study on the effect of CGF on osteogenesis in the rat mandibular symphysis

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