单细胞测序和空间转录组技术在口腔医学的应用进展

doi:10.13591/j.cnki.kqyx.2024.03.010

Abstract

Abstract:

The application of single-cell sequencing and spatial transcriptome technology in life science field has been a hot research topic in recent years. By combining single-cell sequencing and spatial transcriptome technology, it can reveal information on specific spatial location of cells at single-cell level to further investigate the mechanism of disease development and disease prevention and treatment. In the field of dentistry, it has been applied to the study on oral tissue stem cells, oral histogenesis and development, oral microbiology and various oral diseases. This paper describes the progress of single-cell sequencing and spatial transcriptome technology in stomatology and looks into the prospect of their combined application, in order to provide new ideas for the diagnosis and treatment of oral diseases.

Key words: single-cell RNA sequencing, spatial transcriptomics, stomatology, bioinformatic analysis

CLC Number:

R349.8

ZHANG Xiatong, WU Wenzhi, CHEN Zhuo. Progress of single-cell RNA sequencing and spatial transcriptomics in stomatology[J]. Stomatology, 2024, 44(3): 214-221.

Figures/Tables 3

References 57

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原理与特点	单细胞测序	空间转录组
原理	通过微流控,微液滴,微孔技术等将单个细胞遗传物质均匀扩增、标记建库后进行测序,主要流程为单细胞分离与标记、基因组扩增、高通量测序和数据分析等	基于一种特殊化设计的样本捕获芯片,每个捕获点含有数百万探针,当组织切片透化贴在芯片上时,这些探针捕获被释放的mRNA,进行后续的文库构建与测序
特点	①精确度高,达到单细胞水平,但失去细胞原始空间位置 ②组学信息丰富,可获得单细胞内多组学信息	①同时保留细胞的空间位置信息与转录信息,但难以达到单细胞精度 ②具有可视化,将基因表达谱映射到相应的组织形态中

Progress of single-cell RNA sequencing and spatial transcriptomics in stomatology

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