SEMA4D修饰钛表面通过巨噬细胞调控内皮细胞功能的研究

doi:10.13591/j.cnki.kqyx.2024.02.001

Abstract

Abstract:

Objective To explore the direct impact of SEMA4D-modified titanium surface on macrophages and the indirect regulatory effect on endothelial cell functions through macrophages. Methods Pure titanium specimens were polished and cleaned, and SEMA4D coatings were prepared using alkali heat treatment and self-assembly techniques. The experimental group consisted of titanium specimens coated with SEMA4D solutions at concentrations of 25, 50, and 100 ng/mL, with smooth titanium(Ti)specimens as the control group. The surface micromorphology and hydrophilicity of titanium in each group were analyzed by scanning electron microscopy and a contact angle meter. Macrophages were introduced onto the surface of each group, and the resulting supernatant was used to culture endothelial cells. The proliferative activity of the cells was measured using CCK-8 assay. The inflammatory expression and VEGF expression of macrophages were evaluated using real-time quantitative PCR. The adhesion and migration abilities of endothelial cells were determined using the fluorescent staining and scratch assay. Results The SEMA4D-modified titanium surface showed a porous microstructure covered by an organic film, which was non-cytotoxic. This surface reduced macrophage inflammatory expression, up-regulated VEGF expression and promoted endothelial cells’ adhesion and migration. Conclusion SEMA4D modified titanium surface can reduce inflammatory response and enhance the motor function of endothelial cells, thereby obtaining a physiological microenvironment conducive to soft tissue repairing.

Key words: SEMA4D, titanium surface, macrophage, endothelial cell

CLC Number:

R783.1

ZHOU Jieyi, ZHANG Jianlan, XIE Lingling, LIU Yao, QIU Jing. Study on SEMA4D modified titanium surface modulating endothelial cell functions via macrophages[J]. Stomatology, 2024, 44(2): 81-87.

Figures/Tables 8

Tab.1

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

References 33

[1]	Pobloth AM, Checa S, Razi H, et al. Mechanobiologically optimized 3D titanium-mesh scaffolds enhance bone regeneration in critical segmental defects in sheep[J]. Sci Transl Med, 2018, 10(423):eaam8828. doi: 10.1126/scitranslmed.aam8828
[2]	Li J, Cui XL, Hooper GJ, et al. Rational design, bio-functionalization and biological performance of hybrid additive manufactured titanium implants for orthopaedic applications: A review[J]. J Mech Behav Biomed Mater, 2020, 105: 103671. doi: 10.1016/j.jmbbm.2020.103671
[3]	Kaur M, Singh K. Review on titanium and titanium based alloys as biomaterials for orthopaedic applications[J]. Mater Sci Eng C Mater Biol Appl, 2019, 102: 844-862. doi: 10.1016/j.msec.2019.04.064
[4]	Arita S, Gonda T, Togawa H, et al. Influence of mandibular distal extension implant-supported removable partial dentures on the force exerted on maxillary anterior teeth[J]. J Prosthodont Res, 2021, 65(4):541-545. doi: 10.2186/jpr.JPR_D_20_00077
[5]	Arita S, Gonda T, Togawa H, et al. Influence of mandibular free-end partial edentulism on the force exerted on maxillary anterior teeth[J]. J Prosthodont Res, 2020, 64(4):454-459. doi: S1883-1958(19)30265-8 pmid: 32061570
[6]	Brånemark PI. Osseointegration and its experimental background[J]. J Prosthet Dent, 1983, 50(3):399-410. doi: 10.1016/s0022-3913(83)80101-2 pmid: 6352924
[7]	Chackartchi T, Romanos GE, Sculean A. Soft tissue-related complications and management around dental implants[J]. Periodontol 2000, 2019, 81(1):124-138. doi: 10.1111/prd.12287 pmid: 31407443
[8]	Serichetaphongse P, Chengprapakorn W, Thongmeearkom S, et al. Immunohistochemical assessment of the peri-implant soft tissue around different abutment materials: A human study[J]. Clin Implant Dent Relat Res, 2020, 22(5):638-646. doi: 10.1111/cid.2020.v22.5
[9]	Akagawa Y, Hashimoto M, Kondo N, et al. Tissue reaction to implanted biomaterials[J]. J Prosthet Dent, 1985, 53(5):681-686. pmid: 3858534
[10]	Israël A, Le Bail O, Hatat D, et al. TNF stimulates expression of mouse MHC class I genes by inducing an NF kappa B-like enhancer binding activity which displaces constitutive factors[J]. EMBO J, 1989, 8(12):3793-3800. doi: 10.1002/j.1460-2075.1989.tb08556.x pmid: 2555174
[11]	Moreira HR, Raftery RM, da Silva LP, et al. In vitro vascularization of tissue engineered constructs by non-viral delivery of pro-angiogenic genes[J]. Biomater Sci, 2021, 9(6):2067-2081. doi: 10.1039/D0BM01560A
[12]	Zou T, Dissanayaka WL, Jiang S, et al. Semaphorin 4D enhances angiogenic potential and suppresses osteo-/ odontogenic differentiation of human dental pulp stem cells[J]. J Endod, 2017, 43(2):297-305. doi: 10.1016/j.joen.2016.10.019
[13]	Shi W, Kumanogoh A, Watanabe C, et al. The class IV semaphorin CD100 plays nonredundant roles in the immune system: Defective B and T cell activation in CD100-deficient mice[J]. Immunity, 2000, 13(5):633-642. doi: 10.1016/s1074-7613(00)00063-7 pmid: 11114376
[14]	Liu Y, Zhang WS, Tang ZH, et al. Anti-inflammatory effects of the immobilization of SEMA4D on titanium surfaces in an endothelial cell/macrophage indirect coculture model[J]. Biomed Mater, 2021, 17(1): 015005. doi: 10.1088/1748-605X/ac3620
[15]	Hotchkiss KM, Reddy GB, Hyzy SL, et al. Titanium surface characteristics, including topography and wettability, alter macrophage activation[J]. Acta Biomater, 2016, 31: 425-434. doi: S1742-7061(15)30241-5 pmid: 26675126
[16]	Hong GY, Liao MY, Wu T, et al. Improving osteogenic activity of Y-TZP(Yttria-stabilized tetragonal zirconia polycrystal)surfaces by grafting of silanes with different end groups[J]. Appl Surf Sci, 2021, 570: 151144. doi: 10.1016/j.apsusc.2021.151144
[17]	Sun H\|, Chen RC, Li A, et al. Immobilization of bovine serum albumin on poly(ether ether ketone)for surface biocompatibility improvement[J]. Chem Res Chin Univ, 2012, 28(2):353-357.
[18]	Lin DJ, Fuh LJ, Chen WC. Nano-morphology, crystallinityand surface potential of anatase on micro-arc oxidized titanium affect its protein adsorption, cell proliferation and cell differentiation[J]. Mater Sci Eng C Mater Biol Appl, 2020, 107: 110204. doi: 10.1016/j.msec.2019.110204
[19]	Im JS, Choi H, An HW, et al. Effects of surface treatment method forming new nano/micro hierarchical structures on attachment and proliferation of osteoblast-like cells[J]. Materials, 2023, 16(16):5717. doi: 10.3390/ma16165717
[20]	Tzoneva R, Faucheux N, Groth T. Wettability of substrata controls cell-substrate and cell-cell adhesions[J]. Biochim Biophys Acta, 2007, 1770(11):1538-1547. doi: 10.1016/j.bbagen.2007.07.008 pmid: 17804166
[21]	Kuklina E. Semaphorin 4D as a guidance molecule in the immune system[J]. Int Rev Immunol, 2021, 40(4):268-273. doi: 10.1080/08830185.2021.1905807
[22]	Wang L, Li XF, Song Y, et al. The emerging roles of semaphorin4D/CD100 in immunological diseases[J]. Biochem Soc Trans, 2020, 48(6):2875-2890. doi: 10.1042/BST20200821
[23]	Delaire S, Billard C, Tordjman R, et al. Biological activity of soluble CD100. II. Soluble CD100, similarly to H-SemaIII, inhibits immune cell migration[J]. J Immunol, 2001, 166(7):4348-4354. doi: 10.4049/jimmunol.166.7.4348 pmid: 11254688
[24]	Chabbert-de Ponnat I, Marie-Cardine A, Pasterkamp RJ, et al. Soluble CD100 functions on human monocytes and immature dendritic cells require plexin C1 and plexin B1, respectively[J]. Int Immunol, 2005, 17(4):439-447. pmid: 15746246
[25]	Chen Y, ZhangL, Lv R, et al. Overexpression of Semaphorin4D indicates poor prognosis and prompts monocyte differentiation toward M2 macrophages in epithelial ovarian cancer[J]. Asian Pac J Cancer Prev, 2013, 14(10):5883-5890. doi: 10.7314/APJCP.2013.14.10.5883
[26]	Cui YY, Zhou F, Bai LH, et al. SEMA4D-heparin complexes immobilized on titanium surfaces have anticoagulant, cell-migration-promoting, and immunoregulatory effects[J]. ACS Biomater Sci Eng, 2018, 4(5):1598-1608.
[27]	Kikutani H, Kumanogoh A. Semaphorins in interactions between T cells andantigen-presenting cells[J]. Nat Rev Immunol, 2003, 3(2):159-167. pmid: 12563299
[28]	Alig SK, Stampnik Y, Pircher J, et al. The tyrosine phosphatase SHP-1 regulates hypoxia inducible factor-1α(HIF-1α)protein levels in endothelial cells under hypoxia[J]. PLoS One, 2015, 10(3):e0121113. doi: 10.1371/journal.pone.0121113
[29]	Berglundh T, Abrahamsson I, Welander M, et al. Morphogenesis of the peri-implant mucosa: An experimental study in dogs[J]. Clin Oral Implants Res, 2007, 18(1):1-8.
[30]	Zhang M, Gao JN, Zhao XY, et al. p38α in macrophages aggravates arterial endothelium injury by releasing IL-6 through phosphorylating megakaryocytic leukemia 1[J]. Redox Biol, 2021, 38: 101775. doi: 10.1016/j.redox.2020.101775
[31]	Song F, Koo H, Ren D. Effects of material properties on bacterial adhesion and biofilm formation[J]. J Dent Res, 2015, 94(8):1027-1034. doi: 10.1177/0022034515587690 pmid: 26001706
[32]	Singh AV, Vyas V, Patil R, et al. Quantitative characterization of the influence of the nanoscale morphology of nanostructured surfaces on bacterial adhesion and biofilm formation[J]. PLoS One, 2011, 6(9):e25029. doi: 10.1371/journal.pone.0025029
[33]	Whitehead KA, Rogers D, Colligon J, et al. Use of the atomic force microscope to determine the effect of substratum surface topography on the ease of bacterial removal[J]. Colloids Surf B Biointerfaces, 2006, 51(1):44-53. doi: 10.1016/j.colsurfb.2006.05.003

基因名称	引物序列
TNF-α	Forward:5'-GCAAAGGGAGAGTGGTCA-3' Reverse:5'-CTGGCTCTGTGAGGAAGG-3'
IL-1β	Forward:5'-CTCCTGGTATGAAGTGGCAAATC-3' Reverse:5'-ACAAGGCTGCCCCGACTAT-3'
IL-6	Forward:5'-AGGCATAACGCACTAGGTTT-3' Reverse:5'-ACAGAAGGAGTGGCTAAGGA-3'
VEGF	Forward:5'-TAGAGTACATCTTCAAGCCGTC-3' Reverse:5'-CTTTCTTTGGTCTGCATTCACA-3'
GAPDH	Forward:5'-AACTTTGGCATTGGGAAGG-3' Reverse:5'-ACACATTGGGGTAGGAACA-3'

Study on SEMA4D modified titanium surface modulating endothelial cell functions via macrophages

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 33

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FENG Zehua, QIU Shuang, XU Xuanwen, ZHENG Kai, XU Yan. In vitro anti-inflammatory effects of mesoporous bioactive glasses/polycaprolactone fibrous bone tissue engineering scaffolds [J]. Stomatology, 2023, 43(7): 592-599.
[2]	ZHAO Wei,LIU Jinming,YANG Leiting,SHEN Ming,ZHANG Jinglu. Study on the role of human amniotic mesenchymal stem cell-derived exosomes cultured under hypoxic condition in delaying vascular aging [J]. Stomatology, 2023, 43(6): 494-499.
[3]	SUN Dian, XIAO Yijing, SHEN Ming. Study on the effect of circBIRC6 on promoting angiogenesis [J]. Stomatology, 2022, 42(6): 508-514.
[4]	SU Shan, ZHU Wenqing, QIU Jing. The in vitro study on biological behaviors of endothelial cells on the titanium surface modified with zinc-containing nanowires [J]. Stomatology, 2022, 42(5): 404-410.
[5]	YUAN Ying, HUANG Tianyu, WANG Haochen, GONG Ping, XIANG Lin. The role of bone immune microenvironment in implant osseointegration [J]. Stomatology, 2022, 42(12): 1109-1112.
[6]	BI Xiaoming, WANG Wen, GAO Qian, YUAN Changyong. Construction of an indirect co-culture system based on bioprinting technology [J]. Stomatology, 2022, 42(11): 974-978.
[7]	ZHANG Xue, XU Zhaoying, JIANG Pengfei, PAN Shuang. Comparative study on the proliferation and migration of umbilical vein endothelial cells-derived exosome and endothelial progenitor cells-derived exosome on human dental pulp stem cells [J]. Stomatology, 2022, 42(11): 979-983.
[8]	. A preliminary study of polarization of bone marrow-derived macrophages stimulated by porphyromonas gingivalis in db/db mice [J]. , 2020, 40(1): 13-16.
[9]	Hui XUE. Research progress of biological influencing factors of fracture healing [J]. , 2018, 38(11): 1043-1047.
[10]	. Research progress of effects of macrophages on the osteogenic differentiation of mesenchymal stem cells and the underneath mechanisms [J]. , 2018, 38(11): 1026-1030.
[11]	. Studies on the influence of P.g-LPS on the expression of MMP-9 in HUVECS [J]. , 2017, 37(5): 394-397.
[12]	. The effect of porphyromonas gingivalis infection on the metabolism of cholesterol in THP-1-derived macrophage [J]. , 2015, 35(6): 425-428.
[13]	. Effects of tripterygium wilfordii polyglycosidium on the cytotoxicity function of mononuclear-macrophages in acid environment of carcinoma of tongue [J]. , 2015, 35(6): 429-432.
[14]	. Effect of Tamibarotene on P.gingivalis antigen-stimulated RAW264.7 cells [J]. , 2015, 35(5): 349-353.
[15]	. Behavior of calcium and phosphate ions releasing from calcium phosphate cement in initial time and the influence of extraction of calcium phosphate cement on the proliferation capacity of RAW264.7 [J]. , 2015, 35(3): 161-164.