重度牙周炎患者lncRNA表达谱分析及其调控机制

doi:10.13591/j.cnki.kqyx.2026.10.007

Abstract

Abstract:

Objective To analyze the long non-coding RNA (lncRNA) expression profile in patients with severe periodontitis and explore its potential regulatory mechanisms. Methods Gingiva samples were collected from five patients who underwent tooth extraction due to severe periodontitis (test group) and simultaneous extraction of impacted third molars (control group) at the Department of Oral and Maxillofacial Surgery, Affiliated Stomatological Hospital of Suzhou Vocational Health College, between November 2024 and December 2024. High-throughput sequencing was performed to identify differentially expressed lncRNAs and mRNAs. Target genes of these lncRNAs were further screened within these mRNAs, followed by GO and KEGG functional enrichment analysis to investigate the potential regulatory roles of these lncRNAs in severe periodontitis. Results High-throughput sequencing results revealed 1 473 significantly differentially expressed lncRNAs and 7 222 differentially expressed mRNAs. GO enrichment analysis indicated that the differentially expressed lncRNAs may be involved in the pathological process of severe periodontitis by regulating biological processes such as apoptosis, adaptive immune response and endoplasmic reticulum stress. KEGG pathway analysis showed that these lncRNAs might influence the development and progression of severe periodontitis through pathways including leukocyte transendothelial migration, Fcγ receptor-mediated phagocytosis, arachidonic acid metabolism, drug metabolism-cytochrome P450, and transcriptional misregulation in cancer. Conclusion The differentially expressed lncRNAs in patients with severe periodontitis may contribute to the pathogenesis and progress by regulating molecular mechanisms involved in adaptive immune response, apoptosis, and endoplasmic reticulum stress.

Key words: periodontitis, high-throughput sequencing, lncRNA, expression profile, bioinformatics analysis

CLC Number:

R781.4

ZHU Wenwei, LI Hongwei, WANG Ying, LI Wanlin, ZHENG Menglu, WANG Yucong, SANG Lei. Analysis of lncRNA expression profile and its regulatory mechanism in patients with severe periodontitis[J]. Stomatology, 2026, 46(10): 763-770.

Figures/Tables 15

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基因名称	引物序列(5'—3')
GAPDH	F:GGCATGGCCTTCCGTGTCCCC R:CACCCTGTTGCTGTAGCCAAATTC
LPO	F:AAAGACCAGCTCCTCCAAGC R:TGGTAGTCTGCGCTCTTGTG
PLEC	F:AGAGGCACATCAGTGACCTGTA R:ATCATTCCTGATGTTCACCAGCTTC
SLC6A6	F:AGACAACAGACACGCGAGGT R:GGCCATCTCCTTGGTTTGGGA
FZD10	F:GCTCAAGTGCTCCCCGATTA R:GCCTCCATGCACAGGTAGTT
ATP2C1	F:CAATGGATGTTCAGCAGCTTTCA R:AGTCTGGCCCATCGCAACTC
PCAT19	F:ACCCTGCCCTTAGTCAAATC R:TGGAATCCCACACTGTTACC
FENDRR	F:CTCCCGTGGAAGCCATTTCT R:CCTCTGGCTGCGTTTTTCAC
LINC00968	F:GGGTAACTTCAGGTGGAGCC R:ACACGAAAGGCTGGAAGTGT
TPT1-AS1	F:GGGGTGAGCTCGTCGAAGTTCT R:GGGCTGCTGCTGGCGCTGGT
IL12A-AS1	F:TGCCCATGAAGTTGCCTCTG R:CCAGCAGCTTTGATCCTTGG

基因名称	\|log₂FC\|
LINC01484	10.52
LINC02362	9.10
IQCH-AS1	6.46
LINC00662	6.22
PCAT19	6.07
FENDRR	5.83
LINC00968	5.63
IGHV3-64D	5.59
LASTR	5.57
ATP2B4	5.32

基因名称	\|log₂FC\|
LINC02487	12.37
LINC01475	8.54
RPS6P15	8.30
MAP3K9-DT	7.28
LINC01697	6.79
UMPS	6.60
TPT1-AS1	6.48
IL12A-AS1	6.36
MAGI2-AS3	6.30
FAM88B	6.26

基因名称	\|log₂FC\|
RSBN1	22.79
MYO6	22.59
DDX5	22.56
ATP2C1	22.33
ZMYND8	22.26
BICRAL	22.26
SLC6A6	22.08
SLC35B1	21.92
GTPBP2	21.91
PLEC	21.78

基因名称	\|log₂FC\|
ACTN4	24.63
FZD10	24.48
AMBN	24.23
PRR27	24.02
ZG16B	23.99
OBSCN	23.73
LPO	23.72
NUMB	23.43
TASOR2	23.33
ELAPOR1	23.21

Analysis of lncRNA expression profile and its regulatory mechanism in patients with severe periodontitis

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