不同冲洗剂联合超声荡洗对根管壁清洁效果及牙本质结构影响的体外研究

doi:10.13591/j.cnki.kqyx.2025.03.008

Abstract

Abstract:

Objective To evaluate the effect of sodium hypochlorite and Ultra Clean on root canal cleaning and dentin structure. Methods A total of 129 single canal isolated teeth were collected and root canal was prepared to M # with Waveone Gold nickel titanium file. After preparation, they were divided into 3 groups by random number table according to irrigant:group A sodium hypochlorite + normal saline, group B sodium hypochlorite +EDTA, and group C sodium hypochlorite +Ultra Clean. The dye was stained with 1% methylene blue solution, and the penetration depth and range of the dye solution were observed under a type microscope. The removal of debris and stain layer in the root canal was observed by digital camera and scanning electron microscope. The change of dentin microstructure was analyzed by Fourier infrared spectrometer. Results In the upper middle part of the root canal, the penetration depth and range of group C were greater than those of groups A and B (P<0.05); the percentage of debris in group C was lower than that of groups A and B (P<0.05), and the stain layer score in groups B and C was lower than that of group A (P<0.05). In the lower root canal segment, groups B and C were superior to group A in the evaluation of permeability, debris and stain removal (P<0.05). The demineralization ability and exposure of dentin collagen fiber in group C were higher than those in group A and blank group (P<0.05). Conclusion Sodium hypochlorite combined with Ultra Clean is considered to have good cleaning effect and strong demineralization ability, but it may slightly increase the exposure of organic matter such as collagen fiber.

Key words: root canal irrigation, smear layer, dentin

CLC Number:

R781.05

SHUAI Bingjie, ZHANG Hao, ZHOU Yongqing. In vitro study on cleaning effect of different rinsing agents combined with ultrasonic swaging on root canal wall and dentin microstructure[J]. Stomatology, 2025, 45(3): 204-209.

Figures/Tables 10

Fig.1

Tab.1

Penetration range of flushing solutions in each group $\bar{x}$ ±s,%"

组别	根上段	根中段	根下段
A	10.45±1.91^c	7.96±1.15^c	4.61±1.20^b
B	12.92±2.05^b	10.94±1.18^b	6.10±0.57^a
C	14.24±1.99^a	12.82±1.02^a	6.76±0.86^a

Tab.1

Tab.2

Penetration depth of flushing solution in each group $\bar{x}$ ±s,μm"

组别	根上段	根中段	根下段
A	515.27±30.58^c	395.80±42.88^c	163.62±36.07^b
B	617.36±49.84^b	464.89±26.82^b	209.28±16.52^a
C	727.35±51.56^a	516.43±20.45^a	223.01±14.75^a

Tab.2

Fig.2

Tab.3

Percentage of residual debris in the root canal $\bar{x}$ ±s,%"

组别	根上段	根中段	根下段
A	6.34±0.30^a	7.68±0.74^a	12.01±1.86^a
B	5.59±0.41^b	6.21±0.32^b	8.79±0.77^b
C	4.00±0.58^c	5.13±0.20^c	8.59±0.70^b

Tab.3

Fig.3

Tab.4

Root canal smear layer scores for each group $\bar{x}$ ±s"

组别	根上段	根中段	根下段
A	1.56±0.51^a	2.31±0.60^a	2.63±0.89^a
B	0.81±0.75^b	1.44±0.63^b	1.75±0.68^b
C	0.69±0.70^b	0.94±0.77^b	1.69±0.48^b

Tab.4

Fig.4

Fig.5

Tab.5

Ratio of Fourier transform infrared spectra of each group $\bar{x}$ ±s"

组别	碳酸盐v₂峰/磷酸盐v₃峰	酰胺Ⅰ峰/磷酸盐v₃峰
空白对照组	0.37±0.02^b	0.38±0.02^b
A	0.35±0.03^b	0.29±0.09^c
B	0.63±0.05^a	0.65±0.05^a
C	0.67±0.04^a	0.68±0.05^a

Tab.5

References 22

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In vitro study on cleaning effect of different rinsing agents combined with ultrasonic swaging on root canal wall and dentin microstructure

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