应力介导下兔颞下颌关节盘前移位NF-κB及其下游炎症因子的表达

doi:10.13591/j.cnki.kqyx.2025.05.003

摘要/Abstract

摘要：

目的通过建立颞下颌关节盘前移位动物模型,采用苏木精-伊红(HE)染色、免疫组织化学染色及免疫荧光染色等方法观察在应力作用下兔颞下颌关节盘前移位髁突软骨病理变化。方法选取30只成长期新西兰大白兔,随机将其分为Sham组及4组实验组,每组6只。Sham组为假手术组;实验组建立兔颞下颌关节盘前移位动物模型,根据术后处理因素不同又分为Model组(术后不作处理)、2-APB(TRPM7抑制剂)组、PDTC(NF-κB特异性抑制剂)组、Diclofenac(双氯芬酸)组。术后2周处死实验动物,制作髁突软骨病理切片,HE染色法观察髁突软骨病理变化,免疫组织化学染色法、免疫荧光染色法观察NF-κB及炎性因子的表达。结果 ①HE染色见2周时髁突软骨病理变化明显;相比于2-APB组、PDTC组、Diclofenac组,Model组病理变化更为明显。②免疫组织化学染色显示:与Sham组相比,Model组TRPM7、NF-κB、IL-1、IL-6、TNF-α、MMP-3的表达升高,加入2-APB、PDTC或diclofenac治疗后TRPM7、NF-κB、IL-1、IL-6、TNF-α、MMP-3的表达降低。③免疫荧光染色显示,与Sham组相比,Model组NF-κB的表达升高,加入2-APB、PDTC或diclofenac治疗后NF-κB的表达降低。结论 ①在应力作用下髁突骨质早期即发生了改建,且在一定时间内髁突软骨细胞凋亡随加载时间的延长呈上升趋势。②diclofenac、2-APB及PDTC均能减轻髁突细胞凋亡,炎性因子在髁突软骨细胞的凋亡过程中也发挥了重要作用。

关键词: 颞下颌关节盘前移位, 应力, 细胞凋亡, Ca²⁺, NF-κB

Abstract:

Objective To establish an animal model of anterior displacement of the temporomandibular joint disc in rabbits and observe the pathological changes of condylar cartilage under stress by using HE staining, immunohistochemical staining and immunofluorescence staining. Methods Thirty adult New Zealand white rabbits were randomly divided into a Sham operation group and four experimental groups, with six rabbits in each group. The Sham operation group underwent a sham operation, while the experimental groups were used to establish an animal model of anterior displacement of the temporomandibular joint disc. The experimental groups were further divided into a model group (no postoperative treatment), a 2-APB (TRPM7 inhibitor) group, a PDTC (NF-κB specific inhibitor) group, and a Diclofenac group. Two weeks after the operation, the experimental animals were sacrificed, and pathological sections of the condylar cartilage were made. HE staining was used to observe the pathological changes of the condylar cartilage, and immunohistochemical staining and immunofluorescence staining were used to observe the expression of NF-κB and its inflammatory factors. Results ① HE staining showed that the pathological changes of the condylar cartilage were obvious at the 2^ndweek. Compared with the 2-APB group, PDTC group, and Diclofenac group, the pathological changes in the model group were more obvious. ② Immunohistochemical staining showed that the expression of TRPM7, NF-κB, IL-1, IL-6, TNF-α and MMP-3 in the model group was higher than that in the sham operation group. After treatment with 2-APB, PDTC or diclofenac, the expression of TRPM7, NF-κB, IL-1, IL-6, TNF-α and MMP-3 decreased. ③ Immunofluorescence staining showed that the expression of NF-κB in the model group was higher than that in the sham operation group. After treatment with 2-APB, PDTC or diclofenac, the expression of NF-κB decreased. Conclusion ① Under stress, the condylar bone undergoes early remodeling, and the apoptosis of condylar chondrocytes increases with the extension of loading time within a certain period. ② Diclofenac, 2-APB and PDTC can all reduce the apoptosis of condylar cells, and inflammatory factors also play an important role in the apoptosis of condylar chondrocytes.

Key words: temporomandibular joint anterior disc displacement, stress, apoptosis, Ca²⁺, NF-κB

中图分类号:

R782.6

孙江伟, 邓海艳, 刘晓天, 殷瑞, 徐江. 应力介导下兔颞下颌关节盘前移位NF-κB及其下游炎症因子的表达[J]. 口腔医学, 2025, 45(5): 335-341.

SUN Jiangwei, DENG Haiyan, LIU Xiaotian, YIN Rui, XU Jiang. Stress-induced anterior displacement of the rabbit temporomandibular joint disc and expression of NF-κB and its downstream inflammatory factors[J]. Stomatology, 2025, 45(5): 335-341.

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