Tideglusib对LPS刺激的人根尖牙乳头干细胞牙/骨向分化的影响

doi:10.13591/j.cnki.kqyx.2022.07.004

Abstract

Abstract: Objective To investigate effects of Tideglusib on the osteo/odontogenic differentiation of stem cells from the apical papilla stimulated by lipopolysaccharide(LPS). Methods Human stem cells from the apical papilla (SCAPs) were isolated and cultured from human dental papilla, and the surface molecules were identified by flow cytometry. CCK-8 kit was used to detect whether Tideglusib affected cell proliferation of SCAPs. The optimal concentration of Tideglusib promoting ALP activity of SCAPs was screened by alkaline phosphatase (ALP) activity and ALP staining. LPS of Escherichia coli were used to create an inflammatory microenvironment. Western blot and RT-qPCR were applied to investigate changes of proteins (OSX, OCN, COL-Ⅰ, DSP, RUNX2) and genes (OSX, OCN, COL-Ⅰ, DSPP, RUNX2) associated with osteo/odontogenic differentiation. Results CCK-8 results showed that Tideglusib with the concentration below 50 nmol/L had no cytotoxin (P>0.05); ALP results showed that the ALP activity of SCAPs stimulated by LPS treated with 1 nmol/L Tideglusib increased the most significantly (P<0.05); Western blot and RT-qPCR showed that the expression of osteo/odontogenic related proteins (OSX、OCN、COL-Ⅰ、DSP、RUNX2) and genes (OSX, OCN, COL-Ⅰ, DSPP, RUNX2) of SCAPs stimulated by LPS treated with 1 nmol/L Tideglusib markedly increased (P<0.05). Conclusion Tideglusib (1 nmol/L) can promote the osteo/odontogenic differentiation ability of SCAPs stimulated by LPS.

Key words: Tideglusib, LPS, stem cells from the apical papilla, differentiation

CLC Number:

R780.2

JIANG Mei, ZHANG Yuerong, SHEN Tianhui, ZHANG Guangdong. Effects of Tideglusib onosteo/odontogenic differentiation of human stem cells from the apical papilla stimulated by LPS[J]. Stomatology, 2022, 42(7): 593-599.

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Effects of Tideglusib onosteo/odontogenic differentiation of human stem cells from the apical papilla stimulated by LPS

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