Abstract:

Objective To prepare and identify the inclusion complex of resveratrol and cyclodextrin, and analyze the impact of the complex on dentin remineralization. Methods Resveratrol and cyclodextrin were used as raw materials to prepare the resveratrol-cyclodextrin inclusion complex using freeze-drying method. The structures of the inclusion complex were characterized by FTIR and TG. Cell cytotoxicity of resveratrol and the inclusion complex was compared using CCK8 assay. SEM was used to analyze the effect of the inclusion complex on dentin remineralization. Results FTIR spectra showed that compared to resveratrol, the inclusion complexes exhibited decreased and disappeared signal intensities in different frequency bands. Thermal analysis results demonstrated that resveratrol rapidly lost weight at 345 ℃, while the cyclodextrin-resveratrol inclusion complex exhibited rapid weight loss at 342 ℃. The characterization confirmed the successful encapsulation of resveratrol within cyclodextrin. CCK8 results indicated that resveratrol exhibited significant cytotoxicity at a concentration of 5 μg/mL, while the complex showed significant cytotoxicity at 10 μg/mL. The cytotoxicity of resveratrol was reduced after inclusion with cyclodextrin. SEM images indicated that, compared to the pure acid-etched group, both resveratrol and inclusion complexes resulted in mineral formation at demineralized dentin areas, with the group of inclusion complex showing more pronounced dentin remineralization. Conclusion Cyclodextrincan successfully form inclusion complexes with resveratrol, and the complexes promote dentin remineralization.

Key words: remineralization, resveratrol, inclusion complex