纳米硅酸镁锂功能化聚己内酯膜对骨髓间充质干细胞介导骨再生修复的作用研究

doi:10.13591/j.cnki.kqyx.2025.08.002

Abstract

Abstract:

Objective To fabricate nanosilicate functionalized polycaprolactone (PCL/LAP) electrospun membrane and evaluate its role in bone marrow mesenchymal stem cells (BMSCs)-induced bone repairing. Methods The PCL/LAP electrospun membranes were fabricated via electrospinning technology and co-cultured with rat BMSCs. The cytocompatibility of the membranes was evaluated through cytoskeleton staining, live/dead cell staining and CCK-8 assay. The migration capacity of BMSCs was assessed using scratch assay, Transwell migration experiments and expression of migration-related genes (Pdgf and Tgfβ) was evaluated by qRT-PCR. The osteogenic differentiation and pro-angiogenesis potential were determined by alkaline phosphatase (ALP) staining, alizarin red staining, expression levels of osteogenesis-related genes (Alp, Col1a1, Runx2, Bglap and Bmp2) and angiogenesis-related genes (Angpt1, Fgf2 and Vegfa) along with RUNX2 protein expression. PCL and PCL/LAP electrospun membranes conditioned medium was subsequently used to stimulate vascular endothelial cells (EAhy926). The expression of angiogenesis-associated genes (KDR, ENOS and HIF1A) was quantified by qRT-PCR. Results BMSCs adhered well to the surface of the PCL/LAP membranes, with no significant impact on cell viability (P>0.05). PCL/LAP membranes not only promoted the proliferation (P<0.05), migration (P<0.05), but also enhanced ALP activity and mineralized nodule formation (P<0.05), increased osteogenic differentiation gene and protein expression (P<0.05) of BMSCs. Moreover, PCL/LAP promoted the expression of angiogenic genes of BMSCs (P<0.05), to indirectly regulate angiogenesis-related gene expression in vascular endothelial cells (P<0.05). Conclusion PCL/LAP electrospun membranes exhibit excellent biocompatibility and can promote proliferation, migration, osteogenic differentiation and BMSC-mediated angiogenic differentiation, showing great potential for bone defect repairing as barrier membrane.

Key words: barrier membrane, polycaprolactone, nanosilicate, bone marrow mesenchymal stem cells, bone regeneration

CLC Number:

R318.08

XIAO Long, HU Weiqiang, LIN Xuxin, HE Mengjiao, LUO Kai, XU Xiongcheng. Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing[J]. Stomatology, 2025, 45(8): 567-575.

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引物名称	序列(5'-3')
GAPDH	F:CGGCAAGTTCAACGGCACAGTCAAGG R:ACGACATACTCAGCACCAGCATCACC
Alp	F:ACAAGGTGGTGGACGGTGAAC R:CGTGAAGCAGGTGAGCCATAGG
Col1a1	F:CAACAGACTGGCAACCTCAAGAAG R:CACAAGCGTGCTGTAGGTGAATC
Bglap	F:GGACCCTCTCTCTGCTCACTCTG R:ACCTTACTGCCCTCCTGCTTGG
Bmp2	F:AAAGCGTCAAGCCAAACACAAAC R:ACATCACTGAAGTCCACATACAAAGG
Pdgf	F:AAAGGCAAGCACCGAAAG R:GAACAACATTATCACTCCAAGG
Runx2	F:ACCAGCAGCACTCCATATCTCTAC R:CTTCCATCAGCGTCAACACCAT
Tgfβ	F:GGACCTCGGCTGGAAGTG R:CCTTGCTGTACTGCGTGTC
Mmp2	F:CCCCTTCACTTTTCTGGGCA R:CATCAGGGGAGGGCCCATAG

引物名称	序列(5'-3')
Vegfa	F:CGTCCTGTGTGCCCCTAAT R:TGGCTTTGGTGAGGTTTGAT
Fgf2	F:CTGGCTATGAAGGAAGATGGAC R:CGGTAAGTGTTGTAGTTATTGGAC
Angpt1	F:TATGGATGTGAATGAAGGAGGATGG R:ACTGCCTCTGACTGGTTATTGC
KDR	F:CGCAGAGTGAGGAAGGAGGAC R:CCGTAGGATGATGACAAGAAGTAGC
ENOS	F:TTGTCTGCGGCGATGTTACC R:GCGTATGCGGCTTGTCACC
HIF1A	F:AGGACACAGATTTAGACTTGGAGATG R:CAGTGGTAGTGGTGGCATTAGC

Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing

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