机械敏感离子通道Piezo1在糖尿病大鼠牙移动过程中的表达和功能研究

doi:10.13591/j.cnki.kqyx.2022.06.002

Abstract

Abstract: Objective To study the expression of Piezo1 on the tension side of periodontal tissues during orthodontic tooth movement in diabetic rats and explore its role in the remodeling of periodontal tissues. Method Sixty male Wistar rats were divided into the control group and the diabetic group. With bilateral incisors as anchors, 40 g stretching force was applied to move the left first molar forward. Rats were sacrificed at 0 day, the 7^th day, and the 14^th day, and the posterior part of the left maxillary was obtained. HE staining, immunohistochemistry and immunofluorescence were used to examine histological changes and expression levels of Piezo1, Osterix and Runx2. Micro-CT was used to detect parameters related to bone tissues on the tension side. Results During orthodontic tooth movement, the expression of Piezo1 was upregulated by the orthodontic force in the control group on the tension side. The expression level of Piezo1, and osteogenic markers Osterix and Runx2 was significantly decreased in the diabetic group compared to the control group. Conclusion The expression of the Piezo1 and osteogenic activity on the tension side are significantly inhibited in the diabetic group during orthodontic movement. Diabetes could prohibit tissue remodeling on the tension side during orthodontic tooth movement.

Key words: orthodontics, tooth movement, Piezo1, diabetes

CLC Number:

R783.5

GUAN Yuzhe, JIANG Yukun, WU Zuping, LI Tiancheng, HU Zhiai, ZOU Shujuan. Expression and function of mechanosensitive ion channel Piezo1 during tooth movement in diabetic rats[J]. Stomatology, 2022, 42(6): 487-493.

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Expression and function of mechanosensitive ion channel Piezo1 during tooth movement in diabetic rats

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