鱼鳞骨粉及Bio-Oss骨粉对骨髓间充质干细胞成骨分化及体内骨再生作用的比较研究

doi:10.13591/j.cnki.kqyx.2026.02.005

Abstract

Abstract:

Objective To evaluate and compare the cytotoxicity, osteogenic differentiation potential and cranial defect repair capacity of fish scale (FS) bone powder versus Bio-Oss bone powder. Methods FS bone powder was prepared, with Bio-Oss bone powder as the positive control. The microstructures of both materials were examined by scanning electron microscopy (SEM). Cytotoxicity of Bio-Oss and FS extracts (12.5-200.0 mg/mL) on rat bone marrow mesenchymal stem cells (BMSCs) was assessed using live/dead cll staining and CCK-8 assays. BMSCs were co-cultured with Bio-Oss and FS powder and osteogenic gene expression (ALP, RUNX2, OPN) was detected by RT-PCR. Osteogenic mineralization capacity was observed by alizarin red staining. Bio-Oss and FS powder was implanted into rat calvarial critical-sized defects. The bone regeneration was analyzed at 8 weeks post-surgery using Micro-CT, hematoxylin-eosin (HE), and Masson’s trichrome staining. Results FS bone powder particles ranged from 200 to 800 μm in diameter, featuring a corrugated front surface, a relatively smooth back surface, and an irregular morphology. Both Bio-Oss and FS extracts showed no cytotoxicity at concentrations ≤50 mg/mL. Bio-Oss and FS significantly promoted the formation of calcified nodules and upregulated osteogenic genes (ALP, RUNX2, OPN)(P<0.05), with FS exhibiting higher RUNX2 and OPN expression than Bio-Oss (P<0.05). Micro-CT analysis revealed that both the Bio-Oss and FS groups exhibited significantly higher bone volume/tissue volume (BV/TV) (P<0.05). Histological analysis with HE and Masson’s trichrome staining demonstrated greater bone regeneration in both the Bio-Oss and FS groups. Conclusion FS demonstrates excellent cytocompatibility and osteogenic differentiation capacity, achieving comparable bone regeneration to Bio-Oss in rats, suggesting potential applications in bone tissue engineering.

Key words: fish scale bone powder, Bio-Oss bone powder, osteogenic differentiation, bone regeneration

CLC Number:

R728.23

WANG Xiaolei, SU Chang, ZHOU Zhongzheng, QIN Di, HUI Guangyan, WANG Zhongshan, WU Guangsheng. A comparative study on fish scales and Bio-Oss bone powder on osteogenic differentiation of bone marrow mesenchymal stem cells and bone regeneration in vivo[J]. Stomatology, 2026, 46(2): 112-117.

Figures/Tables 8

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References 23

[1]	Firdaus Hussin MS, Abdullah HZ, Idris MI, et al. Extraction of natural hydroxyapatite for biomedical applications-A review[J]. Heliyon, 2022, 8(8): e10356. doi: 10.1016/j.heliyon.2022.e10356
[2]	Liu ZH, Shi JN, Chen LY, et al. 3D printing of fish-scale derived hydroxyapatite/chitosan/PCL scaffold for bone tissue engineering[J]. Int J Biol Macromol, 2024, 274(Pt 2): 133172. doi: 10.1016/j.ijbiomac.2024.133172
[3]	黄崇上, 朱慧勇. 载辛伐他汀PCL-Gt/PCL屏障膜对兔颅骨缺损修复作用的研究[J]. 口腔医学, 2021, 41(12): 1062-1067.
[4]	杨婷婷, 邱伟恩, 陈沁怡, 等. 两种材料联合Bio-Oss骨粉在后牙区拔牙位点保存术中的应用效果研究[J]. 中国实用口腔科杂志, 2022, 15(2): 161-166.
[5]	Pon-On W, Suntornsaratoon P, Charoenphandhu N, et al. Hydr-oxyapatite from fish scale for potential use as bone scaffold or regenerative material[J]. Mater Sci Eng C, 2016, 62: 183-189. doi: 10.1016/j.msec.2016.01.051
[6]	Su SL, Wang RD, Bai JW, et al. Novel decellularization scheme for preparing acellular fish scale scaffolds for bone tissue engineering[J]. ACS Omega, 2025, 10(1): 230-238. doi: 10.1021/acsomega.4c05096 pmid: 39829519
[7]	Wu W, Zhou ZZ, Sun GH, et al. Construction and characterization of degradable fish scales for enhancing cellular adhesion and potential using as tissue engineering scaffolds[J]. Mater Sci Eng C Mater Biol Appl, 2021, 122: 111919.
[8]	Qin D, You XG, Wang HN, et al. Natural micropatterned fish scales combing direct osteogenesis and osteoimmunomodulatory functions for enhancing bone regeneration[J]. Compos Part B Eng, 2023, 255: 110620. doi: 10.1016/j.compositesb.2023.110620
[9]	Wu GS, Feng C, Hui GY, et al. Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles[J]. Carbohydr Polym, 2016, 138: 49-58. doi: 10.1016/j.carbpol.2015.11.044
[10]	Milhan NVM, Carvalho ICS, do Prado RF, et al. Analysis of indicators of osteogenesis, cytotoxicity and genotoxicity of an experimental β-TCP compared to other bone substitutes[J]. Acta Sci Health Sci, 2017, 39(1): 97. doi: 10.4025/actascihealthsci.v39i1.29788
[11]	贾琰, 张保荣. Regesi再生硅、Bio-Oss骨粉对成骨细胞增殖、成骨分化作用的影响[J]. 中国医药导报, 2020, 17(3): 13-16.
[12]	Qiu X, Feng C, Wang WX, et al. Copper-deposited diatom-biosilica enhanced osteogenic potential in periodontal ligament stem cells and rat cranium[J]. J Biomed Mater Res B Appl Biomater, 2023, 111(6): 1286-1298. doi: 10.1002/jbm.b.v111.6
[13]	肖隆, 胡伟强, 林旭馨, 等. 纳米硅酸镁锂功能化聚己内酯膜对骨髓间充质干细胞介导骨再生修复的作用研究[J]. 口腔医学, 2025, 45(8): 567-575.
[14]	Wu GS, Feng C, Quan JJ, et al. In situ controlled release of stromal cell-derived factor-1α and antimiR-138 for on-demand cranial bone regeneration[J]. Carbohydr Polym, 2018, 182: 215-224. doi: 10.1016/j.carbpol.2017.10.090
[15]	Cheng YZ, Dong X, Shi J, et al. Immunomodulation with M2 macrophage-derived extracellular vesicles for enhanced titanium implant osseointegration under diabetic conditions[J]. Mater Today Bio, 2025, 30: 101385.
[16]	Parthasarathy S, Arulselvan P, Gosala R, et al. Exploring the wound healing potential of biocompatible nano-hydroxyapatite derived from parrotfish scale (Scarrus ghobban) waste for bone tissue engineering[J]. Biocatal Agric Biotechnol, 2025, 64: 103493. doi: 10.1016/j.bcab.2025.103493
[17]	白景文, 刘丽丽, 朱华. 鱼鳞作为多功能生物材料的应用研究进展[J]. 安徽农业科学, 2024, 52(3): 6-10, 18.
[18]	Wiedmann-Al-Ahmad M, Gutwald R, Gellrich NC, et al. Search for ideal biomaterials to cultivate human osteoblast-like cells for reconstructive surgery[J]. J Mater Sci Mater Med, 2005, 16(1): 57-66. doi: 10.1007/s10856-005-6447-z
[19]	Ball MD, Downes S, Scotchford CA, et al. Osteoblast growth on titanium foils coated with hydroxyapatite by pulsed laser ablation[J]. Biomaterials, 2001, 22(4): 337-347. pmid: 11205437
[20]	路佳佳, 李伟琼, 许敏, 等. BoneCeramic和Bio-Oss骨粉对狗骨髓间充质干细胞体外成骨分化能力影响的比较研究[J]. 中国药理学通报, 2021, 37(4): 511-516.
[21]	Yang WL, Han WQ, He W, et al. Surface topography of hydro-xyapatite promotes osteogenic differentiation of human bone marrow mesenchymal stem cells[J]. Mater Sci Eng C, 2016, 60: 45-53. doi: 10.1016/j.msec.2015.11.012
[22]	Prathibha PM, Thomas NG, Dalvi YB, et al. Fish scale-derived hydroxyapatite for alveolar ridge preservation[J]. Biotechnol Appl Biochem, 2024, 71(6): 1272-1280. doi: 10.1002/bab.2627 pmid: 38951991
[23]	Wang Y, Kong B, Chen X, et al. BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration[J]. J Nanobiotechnology, 2022, 20(1): 444. doi: 10.1186/s12951-022-01646-9

基因	引物序列(5'—3')
ALP	F:GCCTACACGGTCCTCCTATACGG
ALP	R:CACTGCTGACTGCTGCCGATAC
RUNX2	F:AGGCAGTTCCCAAGCATTTCATCC
RUNX2	R:TGGCAGGTAGGTGTGGTAGTGAG
OPN	F:CAGCCGTGGGAAGGACAGTTATG
OPN	R:TCACATCGGAATGCTCATTGCTCTC
GAPDH	F:TGAAGGTCGGAGTCAACGGATTTGGT
GAPDH	R:CATGTGGGCCATGAGGTCCACCAC

A comparative study on fish scales and Bio-Oss bone powder on osteogenic differentiation of bone marrow mesenchymal stem cells and bone regeneration in vivo

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