芍药苷对人颌骨骨髓间充质干细胞体外增殖及成骨分化的影响

doi:10.13591/j.cnki.kqyx.2026.02.006

Abstract

Abstract:

Objective To investigate the effect of paeoniflorin (PF) on the proliferation and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) and its related mechanisms. Methods hJBMMSCs were isolated and cultured by tissue block method, cultured according to experimental requirements, and the effects of different concentrations (1, 5, 10, 50, 100 μmol/L) PF on the proliferation of hJBMMSCs were detected by CCK-8 method. The effects of PF on the osteogenic differentiation of hJBMMSCs and the expression of proteins related to the PI3K/AKT signaling pathway were investigated by alkaline phosphatase (ALP), alizarin red S staining, RT-qPCR and Western blot. Results PF (10 μmol/L) significantly promoted the proliferation of hJBMMSCs, while 100 μmol/L PF had obvious cytotoxicity. Among them, the expression levels of various osteogenic-related mRNA and protein in hJBMMSCs increased significantly under the condition of 10 μmol/L concentration, and the expression level of p-PI3K was up-regulated. After intervention with PI3K inhibitors, the osteogenic capacity of hJBMMSCs decreased significantly. Conclusion PF (10 μmol/L) may promote the osteogenic differentiation of hJBMMSCs by regulating the PI3K/AKT signaling pathway.

Key words: paeoniflorin, human jaw bone marrow mesenchymal stem cells, osteogenic differentiation, PI3K/AKT signaling pathway

CLC Number:

R780.2

LAI Minqin, HU Zonghao, QIN Zishun, YIN Lihua. Effects of paeoniflorin on proliferation and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells in vitro[J]. Stomatology, 2026, 46(2): 118-125.

Figures/Tables 12

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References 28

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基因	正向引物(5'-3')	反向引物(5'-3')
runx2	CTACTATGGCACTTCGTCAGGAT	ATCAGCGTCAACACCATCATT
alp	CTCCTCGGAAGACACTCTGACC	CTGCGCCTGGTAGTTGTTGTG
ocn	AGCCACCGAGACACCATGAG	GCTCCCAGCCATTGATACAGG
gapdh	GGAAGCTTGTCATCAATGGAAATC	TGATGACCCTTTTGGCTCCC

Effects of paeoniflorin on proliferation and osteogenic differentiation of human jaw bone marrow mesenchymal stem cells in vitro

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