咖啡通过抑制Wnt/β-catenin通路介导大鼠骨髓间充质干细胞成骨分化

doi:10.13591/j.cnki.kqyx.2026.05.003

Abstract

Abstract:

Objective To investigate the effect of coffee on the osteogenic differentiation of rat bone marrow mesenchymal stem cells （BMMSCs） and preliminarily elucidate its relationship with the Wnt/β-catenin signaling pathway. Methods Rat BMMSCs were isolated and cultured using the whole bone marrow adherence method. Coffee-conditioned medium was prepared at different concentrations，and the optimal concentration was selected based on alkaline phosphatase （ALP） staining. Changes in osteogenesis-related markers were observed via qRT-PCR and Western blot，and alterations in the gene and protein expression levels of β-catenin were detected. Results Rat BMMSCs were successfully isolated and cultured. ALP activity assay identified 0.1 mg/mL as the optimal concentration of coffee-conditioned medium. Further experiments showed that this concentration had no significant effect on the proliferation of rat BMMSCs during the first 3 days but inhibited their proliferation from Day 5 to 9. Coffee-conditioned medium suppressed the expression of osteogenic markers in rat BMMSCs and concurrently downregulated the expression of proteins related to the Wnt/β-catenin signaling pathway. Conclusion Coffee-conditioned medium can inhibit the osteogenic differentiation of rat BMMSCs，and its mechanism may be associated with the suppression of the Wnt/β-catenin signaling pathway.

Key words: coffee, bone marrow mesenchymal stem cells （BMMSCs）, osteogenic differentiation, Wnt/β-catenin signaling pathway

CLC Number:

R780.1
R36

WANG Jie, LI Na, YU Jinhua. Coffee inhibits osteogenic differentiation of rat bone marrow mesenchymal stem cells via suppression of the Wnt/β-catenin pathway[J]. Stomatology, 2026, 46(5): 337-343.

Figures/Tables 6

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References 21

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基因	引物序列（5'—3'）	GenBank编号
Alp	F：5'-GGAACGGATCTCGGGGTACA-3' R：5'-ATGAGTTGGTAAGGCAGGGT-3'	NM_013059.1
Runx2	F：5'-TTAACGTCAGCAGGAGCAG-3' R：5'-CTTCACCCCCAGGACCAAG-3'	NM_053470.1
Osx	F：5'-GGAGGCACAAAGAAGCCATA-3' R：5'-GGGAAAGGGTGGGTAGTCAT-3'	NM_181374.2
β-catenin	F：5'-GCAGCGACTAAGCAGGAAGG-3' R：5'-TCACCAGCACGAAGGACAGT-3'	NC_086026.1
Gapdh	F：5'-CACTGAGCATCTCCCTCACAA-3' R：5'-GTATTCGAGAGAAGGGAGGGCT-3'	NM_017008.4

Coffee inhibits osteogenic differentiation of rat bone marrow mesenchymal stem cells via suppression of the Wnt/β-catenin pathway

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